Eriksson H, Brown K
Mol Cell Endocrinol. 1976 Feb;4(3):157-65. doi: 10.1016/0303-7207(76)90035-6.
Perfusions with corticosterone, of isolated regenerated livers from adult male rats, subjected to castration, partial hepatectomy and hypophysectomy with or without estradiol treatment during parenchymal regeneration, yielded very similar patterns of biliary steroid metabolites. The degree of steroid conjugation was lower than that seen in livers from normal, untreated, adult male rats. In operated animals, with or without estradiol benzoate treatment, ring A-reduced 20-keto metabolites constituted about 20%, whereas metabolites with a 20-hydroxy group made up approximately 80% of the corticosterone metabolites formed. Furthermore, no 15-hydroxylated metabolites derived from corticosterone, quantitatively the most important compounds in bile from female rats, could be detected in bile from these treated male animals. However, livers from male rats which had been castrated, hepatectomized and treated with estradiol benzoate during liver regeneration, produced 15-hydroxy-tetrahydrocorticosterone to the same extent as female rat livers, when perfused with corticosterone. The results obtained indicate that the effects of estradiol on the induction and differentiation of steroid metabolizing enzymes in the regenerating liver are pronounced and manifested only in the presence of an intact hypophysis.
对成年雄性大鼠进行去势、部分肝切除和垂体切除，在实质再生过程中给予或不给予雌二醇处理，然后将分离出的再生肝脏用皮质酮灌注，结果产生了非常相似的胆汁类固醇代谢物模式。类固醇结合程度低于正常、未处理的成年雄性大鼠肝脏中的结合程度。在接受手术的动物中，无论是否接受苯甲酸雌二醇处理，A环还原的20-酮代谢物约占20%，而具有20-羟基的代谢物约占所形成的皮质酮代谢物的80%。此外，在这些经处理的雄性动物的胆汁中，未检测到来自皮质酮的15-羟基化代谢物（雌性大鼠胆汁中数量上最重要的化合物）。然而，在肝脏再生过程中接受去势、肝切除并经苯甲酸雌二醇处理的雄性大鼠肝脏，在用皮质酮灌注时，产生15-羟基-四氢皮质酮的程度与雌性大鼠肝脏相同。所得结果表明，雌二醇对再生肝脏中类固醇代谢酶的诱导和分化作用显著，且仅在垂体完整的情况下才会表现出来。
