Layne E C, Schultz R D, Thomas L J, Slama G, Sayler D F, Bessman S P
Diabetes. 1976 Feb;25(2):81-9. doi: 10.2337/diab.25.2.81.
A continuous extracorporeal monitoring system for blood glucose employing an electrochemical sensor is described. The sensor, about the size of a nickel, is rapid, is specific for glucose, generates its own power, and consists of two galvanic oxygen electrodes. Over one oxygen electrode is affixed a plastic matrix to which glucose oxidase is covalently bound; a blank matrix is over the other, which serves as a reference. Oxygen is consumed in the glucose-oxidase-containing matrix, decreasing the current from the underlying oxygen electrode. The current decrease is nonlinearly proportional to the glucose concentration. The sensor is clamped between small blocks of plastic fitted with inlet and outlet nipples so that blood pumped from the animal passes over the two electrodes and thence to an automated chemical analysis for comparison. Blood is collected and anticoagulant added in a double-lumen catheter. Blood is withdrawn at the rate of 1 cc. per hour. Results obtained by use of the system in rabbits are reported. The capacity of the system to continuously monitor changes in blood glucose produced by repeated glucose tolerances is shown in hypo-, normo-, and hyperglycemic animals. Some properties of the system and its calibration are discussed.
描述了一种采用电化学传感器的连续体外血糖监测系统。该传感器约为镍币大小，反应迅速，对葡萄糖具有特异性，能自行产生能量，由两个原电池型氧电极组成。在一个氧电极上固定有共价结合葡萄糖氧化酶的塑料基质；另一个电极上覆盖的空白基质用作参考。含葡萄糖氧化酶的基质中氧被消耗，从而降低下方氧电极的电流。电流降低与葡萄糖浓度呈非线性比例关系。传感器夹在装有进出液乳头的小塑料块之间，以便从动物体内泵出的血液流经两个电极，然后进行自动化学分析以作比较。通过双腔导管采集血液并添加抗凝剂。血液以每小时1毫升的速度抽取。报告了该系统在兔子身上使用的结果。该系统在低血糖、正常血糖和高血糖动物中显示出连续监测重复葡萄糖耐量引起的血糖变化的能力。讨论了该系统的一些特性及其校准。
