Damasceno D C, Volpato G T, Sartori T C F, Rodrigues P F, Perin E A, Calderon I M P, Rudge M V C
Experimental Laboratory of Obstetrics, Department of Gynecology and Obstetrics of School of Human Medicine of Botucatu-UNESP, São Paulo State, Brazil.
Phytomedicine. 2002 Oct;9(7):667-72. doi: 10.1078/094471102321616508.
Annona squamosa Linn., family Annonaceae, is said to show varied medicinal effects, including insecticide, antiovulatory and abortifacient. The purpose of present study was to investigate if A. squamosa seed aqueous extract, in doses higher than that popularly used to provoke abortion, interferes with reproductive performance, and to correlate the ingestion of this extract with possible alterations in rat embryonic implantation. Doses of 300 mg/kg (Treated Group I, n = 17) and 600 mg/kg (Treated Group II, n = 12) body wt. were administered by gavage, during days 1 to 5 of pregnancy (preimplantation period). The control group (n = 13) received water in the same manner, during the same period for comparison with experimental groups. The animals were euthanized on day 10 of pregnancy. Treatment of dams during the preimplantation period showed no signs of toxicity, and no alteration in the corpora lutea, implantations and embryo in terms of development numbers. The percentage of preimplantation and postimplantation losses in treated groups I and II did not differ from those of control. Treatment with aqueous extract of A. squamosa seeds caused no morphological change in the endometrium. The absence of morphological alterations in uterine epithelial cells in treated groups I and II permitted a viable embryonic implantation, as verified by the number of embryos in development at day 10 of pregnancy. Thus, A. squamosa seed aqueous extract did not interfere with the reproductive performance of pregnant rats.
番荔枝科的番荔枝据说具有多种药用功效，包括杀虫、抗排卵和堕胎作用。本研究的目的是调查番荔枝种子水提取物在高于常用于引发流产的剂量下，是否会干扰生殖性能，并将这种提取物的摄入与大鼠胚胎着床的可能变化联系起来。在妊娠第1至5天（着床前期），通过灌胃给予300mg/kg体重（处理组I，n = 17）和600mg/kg体重（处理组II，n = 12）的剂量。对照组（n = 13）在同一时期以相同方式给予水，以便与实验组进行比较。在妊娠第10天对动物实施安乐死。在着床前期对母鼠进行处理未显示出毒性迹象，黄体、着床和胚胎在发育数量方面也没有改变。处理组I和II的着床前和着床后损失百分比与对照组没有差异。番荔枝种子水提取物处理未引起子宫内膜形态变化。处理组I和II子宫上皮细胞未出现形态改变，这使得胚胎能够成功着床，这在妊娠第10天发育中的胚胎数量上得到了证实。因此，番荔枝种子水提取物不会干扰怀孕大鼠的生殖性能。
