Batchelor B R, Penner J, Hirsch J, Stern J S
Horm Metab Res. 1976 Jan;8(1):24-33. doi: 10.1055/s-0028-1093687.
Glucose oxidation and incorporation into lipid were measured in epididymal adipose tissues and isolated adipose cells of normal and hypophysectomized rats in an effort to determine whether the acute hypoglycemic effect of a systemic growth hormone (GH) injection was related to alterations in the glucose metabolism of adipose tissue. The rats were fed rat chow or a high sucrose diet and received 100 mug GH intraperitoneally 30 minutes or three and one-half hours before sacrifice. Hypophysectomized rats showed a lower plasma glucose as compared with normal rats on both diets. Thirty minutes after a GH injection there was a further decrease of the plasma glucose which, however, was not present in those rats receiving GH three and one-half hours before sacrifice. Adipose tissues from hypophysectomized rats fed the high sucrose diet showed a blunted insulin sensitivity as compared with normal rats on a similar diet. The insulin sensitivity of these tissues was further decreased 30 minutes after a GH injection. Basal glucose metabolism of isolated adipocytes from hypophysectomized rats, as compared with normal rats, was depressed if they were fed rat chow, was at normal levels if they were fed the high sucrose diet and was increased if they were fed the sucrose diet and received triiodothyronine and cortisone supplements. No manipulations of diet or hormonal treatments made the isolated adipocyte from hypophysectomized rats sensitive to insulin either 30 minutes or three and one-half hours after a GH injection. Since basal glucose utilization is not enhanced by GH injection and both the blunted insulin sensitivity of adipose tissue and the absent insulin sensitivity of adipopocytes would be expected to produce hyperglycemia rather than hypoglycemia, it is concluded that immediate systemic effects of a GH injection on carbohydrate metabolism are not related to changes in glucose metabolism of the peripheral adipose tissues.
为了确定全身注射生长激素（GH）的急性降血糖作用是否与脂肪组织葡萄糖代谢的改变有关，对正常大鼠和垂体切除大鼠的附睾脂肪组织及分离的脂肪细胞中的葡萄糖氧化及脂质掺入情况进行了测定。给大鼠喂食大鼠饲料或高蔗糖饮食，并在处死前30分钟或3.5小时腹腔注射100微克GH。两种饮食条件下，垂体切除大鼠的血糖均低于正常大鼠。注射GH 30分钟后，血糖进一步降低，但在处死前3.5小时注射GH的大鼠中未出现这种情况。与喂食相似饮食的正常大鼠相比，喂食高蔗糖饮食的垂体切除大鼠的脂肪组织胰岛素敏感性降低。注射GH 30分钟后，这些组织的胰岛素敏感性进一步降低。与正常大鼠相比，喂食大鼠饲料的垂体切除大鼠分离的脂肪细胞基础葡萄糖代谢降低；喂食高蔗糖饮食时，基础葡萄糖代谢处于正常水平；喂食蔗糖饮食并补充三碘甲状腺原氨酸和可的松时，基础葡萄糖代谢增加。无论饮食如何调整或进行激素处理，垂体切除大鼠分离的脂肪细胞在注射GH 30分钟或3.5小时后对胰岛素均不敏感。由于注射GH后基础葡萄糖利用并未增强，且脂肪组织胰岛素敏感性降低和脂肪细胞胰岛素不敏感均预期会导致高血糖而非低血糖，因此得出结论，注射GH对碳水化合物代谢的即时全身作用与外周脂肪组织葡萄糖代谢的变化无关。
