Li Ningdong, Li Bin, Sun Xianli, Li Liaoqing, Chen Changxi
Beijing Institute of Ophthalmology, Beijing Tong Ren Ophthalmic Center, Capital University of Medical Sciences, Beijing 100730, China.
Zhonghua Yan Ke Za Zhi. 2002 Nov;38(11):684-7.
To investigate the roles of telomerase activity (TA) and human telomerase RNA (hTR) in tarsal gland carcinoma.
The telomerase repeated amplification protocol (TRAP) was used to demonstrate telomerase activity in 12 tarsal gland carcinomas. In situ hybridization (ISH) was used to demonstrate the expression of hTR in 55 cases of paraffin-embedded tarsal gland carcinoma, and the results were compared with the proliferative index determined by Mib-1 immunolabeling, histological patterns and recurrence of the tumor.
The telomerase activity was revealed in 12 tarsal gland carcinomas with different degrees. The positive expression of hTR was 85.5% (47/55) and observed in tumor cells, but not in the adjacent tissues. The expression of hTR was correlated with the proliferative activity (as assessed by Mib-1 immunolabelling, r = 0.942, P < 0.001) and the differentiation of tarsal gland carcinoma (chi(2) = 17.621, P < 0.001), but no significant relationship with tumor recurrence. The level of hTR expression had a gradually raised tendency along with the decrease of differentiation of tarsal gland carcinoma.
The results suggest that up-regulation of telomerase expression play some roles in tarsal gland carcinogenesis, and the expression of hTR be a useful marker for malignant degree of tarsal gland carcinoma.
探讨端粒酶活性（TA）及人端粒酶RNA（hTR）在睑板腺癌中的作用。
采用端粒酶重复序列扩增法（TRAP）检测12例睑板腺癌组织中端粒酶活性。应用原位杂交（ISH）技术检测55例石蜡包埋的睑板腺癌组织中hTR的表达情况，并与Mib-1免疫标记法检测的增殖指数、组织学类型及肿瘤复发情况进行比较。
12例睑板腺癌组织均检测到不同程度的端粒酶活性。hTR阳性表达率为85.5%（47/55），阳性信号见于肿瘤细胞，而在癌旁组织中未见表达。hTR的表达与睑板腺癌的增殖活性（以Mib-1免疫标记法评估，r = 0.942，P < 0.001）及分化程度（χ2 = 17.621，P < 0.001）相关，但与肿瘤复发无明显关系。hTR表达水平随睑板腺癌分化程度降低呈逐渐升高趋势。
结果提示端粒酶表达上调在睑板腺癌发生过程中起一定作用，hTR的表达可作为睑板腺癌恶性程度的一个有用指标。
