Li Bin, Li Ning-dong, Xu Xiao-lin, Zheng Bang-he, Sun Xian-li, Li Liao-qing, Chen Chang-xi
Department of Ophthalmic Pathology, Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Capital University of Medical Sciences, Beijing 100005, China.
Chin Med J (Engl). 2004 Mar;117(3):445-8.
In humans telomerase is expressed in most cancers and immortal cell lines, and activation of telomerase may play important roles in tumorigenesis and immortalization. This study was to investigate the roles of telomerase activity (TA) and human telomerase RNA (hTR) in sebaceous carcinoma of the eyelid.
The telomerase repeated amplification protocol (TRAP) was used to demonstrate telomerase activity in 12 cases of sebaceous carcinoma of the eyelid. In situ hybridization (ISH) was used to demonstrate the expression of hTR in 55 cases of paraffin-embedded sebaceous carcinoma of the eyelid, and the results were compared with the proliferative index determined by Mib-1 immuno-labeling, histological patterns and recurrence of the tumor.
Different telomerase activity was shown in the 12 cases of sebaceous carcinoma of the eyelid. The positive expression of hTR was 85.5% (47/55) in tumor cells, but not in the adjacent tissues. The positive expression of hTR was correlated with the proliferative activity (as assessed by Mib-1 immunolabelling, r = 0.942, P < 0.001) and the differentiation of sebaceous carcinoma of the eyelid (chi(2) = 17.621, P < 0.001), but not significantly related to tumor recurrence. The level of hTR expression increased with the decrease of differentiation of sebaceous carcinoma of the eyelid.
The results suggest that the up-regulation of telomerase expression plays some roles in tarsal gland carcinogenesis, and the expression of hTR is a useful marker for malignant degree of sebaceous carcinoma of the eyelid.
在人类中，端粒酶在大多数癌症和永生化细胞系中表达，端粒酶的激活可能在肿瘤发生和永生化过程中发挥重要作用。本研究旨在探讨端粒酶活性（TA）和人端粒酶RNA（hTR）在眼睑皮脂腺癌中的作用。
采用端粒酶重复扩增法（TRAP）检测12例眼睑皮脂腺癌中端粒酶活性。采用原位杂交（ISH）检测55例眼睑皮脂腺癌石蜡包埋组织中hTR的表达，并将结果与通过Mib-1免疫标记测定的增殖指数、组织学类型和肿瘤复发情况进行比较。
12例眼睑皮脂腺癌显示出不同的端粒酶活性。hTR在肿瘤细胞中的阳性表达率为85.5%（47/55），而在相邻组织中未检测到。hTR的阳性表达与增殖活性（通过Mib-1免疫标记评估，r = 0.942，P < 0.001）和眼睑皮脂腺癌的分化程度（χ2 = 17.621，P < 0.001）相关，但与肿瘤复发无明显相关性。hTR表达水平随着眼睑皮脂腺癌分化程度的降低而升高。
结果表明端粒酶表达上调在睑板腺癌发生中起一定作用，hTR的表达是眼睑皮脂腺癌恶性程度的一个有用标志物。
