Miguel-Hidalgo José Javier, Wei Jinrong, Andrew Michael, Overholser James C, Jurjus George, Stockmeier Craig A, Rajkowska Grazyna
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, Mississippi, USA.
Biol Psychiatry. 2002 Dec 15;52(12):1121-33. doi: 10.1016/s0006-3223(02)01439-7.
Reductions in glial density and enlargement of glial nuclei have been reported in the dorsolateral prefrontal cortex (dlPFC) in mood disorders. In alcohol dependence, often comorbid with depression, it is unclear whether there are changes in the density and size of glial cells in the dlPFC.
The packing density and size of Nissl-stained glial cell nuclei were analyzed postmortem in the cortical layers of the dlPFC from 21 control and 17 alcohol-dependent (Alc) subjects without Wernicke or Korsakoff syndromes. Eight Alc subjects had depressive symptoms. The density of glial cells was measured with a three-dimensional cell counting method, and the areal fraction of glial fibrillary acidic protein immunoreactivity (GFAP) was also determined.
Glial density was reduced by 11-14% in layers V and VI and in all layers combined in the Alc group. The size of glial nuclei was decreased by 3.2% in Alc subjects. The Alc subjects with depressive symptoms showed the lowest values of density and size. There was no difference in GFAP immunoreactivity, although the lowest values were in the Alc group.
Alcohol dependence is characterized by decreases in both density and size of glia in the dlPFC. Glial pathology may be more severe in Alc subjects with depressive symptoms.
在情绪障碍患者的背外侧前额叶皮质(dlPFC)中,已报道出现胶质细胞密度降低和胶质细胞核增大的情况。在常与抑郁症共病的酒精依赖中,dlPFC中胶质细胞的密度和大小是否发生变化尚不清楚。
对21名无韦尼克或科萨科夫综合征的对照受试者和17名酒精依赖(Alc)受试者的dlPFC皮质层进行尸检,分析尼氏染色的胶质细胞核的堆积密度和大小。8名Alc受试者有抑郁症状。采用三维细胞计数法测量胶质细胞密度,并测定胶质纤维酸性蛋白免疫反应性(GFAP)的面积分数。
Alc组V层和VI层以及所有层合并后的胶质细胞密度降低了11%-14%。Alc受试者的胶质细胞核大小减少了3.2%。有抑郁症状的Alc受试者的密度和大小值最低。GFAP免疫反应性无差异,尽管最低值出现在Alc组。
酒精依赖的特征是dlPFC中胶质细胞的密度和大小均降低。有抑郁症状的Alc受试者的胶质细胞病理可能更严重。