Korngold L
Int Arch Allergy Appl Immunol. 1976;50(3):297-308. doi: 10.1159/000231507.
Four out of 18 human liver alkaline phosphatase (AP) preparations reacted with anti-liver and anti-intestinal AP. The liver AP that reacted with anti-intestinal AP, designated as intestine-like AP, was precipitated at 50% ammonium sulfate whereas the major liver AP precipitated at 66% saturation. Gel filtration showed that liver AP, intestine-like AP and intestinal AP contained AP with apparent molecular weights of 130,000 daltons; the intestine-like AP contained a second but smaller component of 70,000-80,000 daltons. AP extracted from intestine also contained this smaller component; its electrophoretic mobility was that of an a2-globulin, whereas that of the intestine-like AP had a mobility of beta-globulin. The similarity of the intestine-like AP to the AP variant found in hepatomas is stressed.
18种人肝碱性磷酸酶（AP）制剂中有4种与抗肝和抗肠AP发生反应。与抗肠AP发生反应的肝AP，被指定为肠样AP，在50%硫酸铵中沉淀，而主要的肝AP在66%饱和度时沉淀。凝胶过滤显示，肝AP、肠样AP和肠AP含有表观分子量为130,000道尔顿的AP；肠样AP含有第二种但较小的70,000 - 80,000道尔顿的成分。从肠中提取的AP也含有这种较小的成分；其电泳迁移率为α2球蛋白的迁移率，而肠样AP的迁移率为β球蛋白的迁移率。强调了肠样AP与在肝癌中发现的AP变体的相似性。
