Kuroume T, Suzuki S, Tomizawa S, Shibasaki M, Nemoto H
Int Arch Allergy Appl Immunol. 1976;50(4):385-401. doi: 10.1159/000231529.
Firstly, it was reported that phytohemagglutinin P (PHA-P) can induce the immediate skin reaction besides the delayed one. 70 out of 80 cases (87.5%) gave positive immediate reactions with 1 mug of PHA-P. There was a close correlation between the radioallergosorbent test (RAST) counts against PHA-P and the total IgE value or erythematous diameter of the immediate skin reactions. Absorption with anti-IgE or PHA-P reduced the RAST counts against PHA-P to 79 and 56% of their initial values, respectively, but did not effect the RAST counts against steptokinase-streptodornase (SK-SD). These results suggest that the immediate skin reaction might be induced by specific IgE antibody against PHA-P. Secondly, using three fractions of PHA-P obtained by Sephadex G-200 gel filtration, skin test and RAST in humans, ACA in rats, and 3H-thymidine incorporation into human leukocytes, were carried out and data obtained that showed some discrepancy between them. That is, on skin testing, it was shown that all of the three fractions (fr.) had the ability to elicit the immediate and delayed skin reaction in humans and rats with the order of fr. 1, 2 and 3, and it was confirmed by RAST. On the other hand, the leukocyte-stimulating activity resided in fr. 1 and 2, while fr. 3, which seemed to be a group of subunits with a molecular weight of around 35,000, had almost no activity. These findings have been discussed in view of the earlier studies on the structure of PHA-P
首先，据报道，植物血凝素P（PHA-P）除了能引起迟发性皮肤反应外，还能引起速发性皮肤反应。80例中有70例（87.5%）对1微克PHA-P产生了阳性速发性反应。针对PHA-P的放射变应原吸附试验（RAST）计数与速发性皮肤反应的总IgE值或红斑直径之间存在密切相关性。用抗IgE或PHA-P吸收后，针对PHA-P的RAST计数分别降至其初始值的79%和56%，但不影响针对链激酶-链道酶（SK-SD）的RAST计数。这些结果表明，速发性皮肤反应可能是由针对PHA-P的特异性IgE抗体诱导的。其次，使用通过Sephadex G-200凝胶过滤获得的PHA-P的三个组分，对人类进行皮肤试验和RAST，对大鼠进行ACA试验，并进行3H-胸腺嘧啶核苷掺入人白细胞的试验，获得的数据显示它们之间存在一些差异。也就是说，在皮肤试验中，结果表明所有三个组分（fr.）都有能力在人类和大鼠中引发速发性和迟发性皮肤反应，顺序为fr.1、2和3，并且通过RAST得到了证实。另一方面，白细胞刺激活性存在于fr.1和2中，而fr.3似乎是一组分子量约为35000的亚基，几乎没有活性。鉴于早期对PHA-P结构的研究，对这些发现进行了讨论。
