Fritze D, Kern D H, Humme J A, Drogemuller C R, Pilch Y H
Int J Cancer. 1976 Jan 15;17(1):138-47. doi: 10.1002/ijc.2910170118.
Two fibrosarcomas of similar histological type, induced in C3Hf mice by either methylcholanthrene or 3,4-benz(a)pyrene, were shown to have individually unique tumor-rejection antigens in classical transplantation-type experiments. By contrast, sera of autochthonous mice, which resisted only transplants of the immunizing sarcoma, were found to contain complement-dependent cytotoxic antibodies, specific for both sarcomas, in vitro. The existence of individually unique as well as common tumor-associated antigens in chemically-induced murine sarcomas is suggested. The private "tumor transplantation-type" antigens elicited tumor rejection responses in vivo. The common tumor-associated antigens, although immunogenic in autochthonous hosts, inducing the production of tumor-specific antibodies, failed to induce transplantation cross-resistance in vivo. This study supports the contention that, in carcinogen-induced murine tumors, and perhaps in human neoplasms as well the evaluation of humoral (and cell-mediated) immune responses in vitro may not reflect tumor rejection-type immune responses in vivo.
在经典的移植型实验中，通过甲基胆蒽或3,4-苯并(a)芘在C3Hf小鼠中诱导产生的两种组织学类型相似的纤维肉瘤，各自具有独特的肿瘤排斥抗原。相比之下，仅能抵抗免疫肉瘤移植的同基因小鼠血清，在体外被发现含有对两种肉瘤均具有特异性的补体依赖性细胞毒性抗体。这表明在化学诱导的小鼠肉瘤中存在个体独特的以及共同的肿瘤相关抗原。个体独特的“肿瘤移植型”抗原在体内引发肿瘤排斥反应。共同的肿瘤相关抗原虽然在同基因宿主中具有免疫原性，可诱导产生肿瘤特异性抗体，但在体内未能诱导移植交叉抗性。这项研究支持了这样一种观点，即在致癌物诱导的小鼠肿瘤中，或许在人类肿瘤中也是如此，体外体液（和细胞介导）免疫反应的评估可能无法反映体内肿瘤排斥型免疫反应。
