Gurevich Pavel, Elhayany Asher, Ben-Hur Herzl, Moldavsky Moisey, Szvalb Sergio, Zandbank Judit, Schneider David F, Zusman Itshak
Laboratory of Experimental Medicine, Institute of Pathology, Assaf Harofeh Medical Center, 12B Hamada Street, Park Rabin, Rehovot 76100, Israel.
Pediatr Dev Pathol. 2003 Jan-Feb;6(1):35-42. doi: 10.1007/s10024-001-0277-x. Epub 2002 Dec 20.
In our previous studies, we described the development of the secretory (mucosal) immune system (SIS) in human fetuses in the second trimester of pregnancy. In the present study, we examined the presence and distribution of components of this system in human embryos and early fetuses in the first trimester. An immunohistochemical study was performed on 17 embryos and 9 fetuses (4 to 12 wk of development) using antibodies against secretory component (SC), joining (J) chain, immunoglobulins (IgA, IgM, IgG), subsets of T and B lymphocytes, and macrophages. Cells positive for SC, J chain, and IgG were found in epithelial tissues from wk 4 of pregnancy. In the internal organs, such as the myocardium and endocardium, capillary endothelium, epithelium of the kidney tubules and some others, only J chain and immunoglobulins were seen. IgA was weakly reactive in tissues where SC and/or J chain were presented. IgM was very weak or absent. Among the cellular components of the SIS, only macrophages were seen in 4-wk-old embryos. CD3+ and CD20+ lymphocytes were found at wk 7 to 8. IgA- and IgM-positive lymphocytes appeared at the end of wk 9. The SIS is widespread in embryonic and early fetal periods and begins to function before the appearance of the common immune system in the developing organism. The first functional components of the SIS, such as IgG and IgA observed in this study, are most probably of maternal origin.
在我们之前的研究中，我们描述了妊娠中期人类胎儿分泌性（黏膜）免疫系统（SIS）的发育情况。在本研究中，我们检查了该系统的组成成分在妊娠早期人类胚胎和早期胎儿中的存在情况及分布。使用针对分泌成分（SC）、连接（J）链、免疫球蛋白（IgA、IgM、IgG）、T和B淋巴细胞亚群以及巨噬细胞的抗体，对17个胚胎和9个胎儿（发育4至12周）进行了免疫组织化学研究。在妊娠第4周的上皮组织中发现了SC、J链和IgG阳性细胞。在内脏器官，如心肌、心内膜、毛细血管内皮、肾小管上皮等组织中，仅可见J链和免疫球蛋白。在存在SC和/或J链的组织中，IgA反应较弱。IgM反应非常弱或未检测到。在SIS的细胞成分中，仅在4周龄的胚胎中发现了巨噬细胞。在第7至8周发现了CD3 +和CD20 +淋巴细胞。IgA和IgM阳性淋巴细胞在第9周结束时出现。SIS在胚胎期和胎儿早期广泛存在，并在发育中的生物体中常见免疫系统出现之前就开始发挥作用。本研究中观察到的SIS的首批功能成分，如IgG和IgA，很可能源自母体。
