Bäck N, Rechardt L, Partanen S
Histochemistry. 1976 Feb 16;46(2):121-30. doi: 10.1007/BF02462736.
Light and electron microscopic histochemical reactions were studied in the cells of pars intermedia of the rat. The possible correlations between enzymatic reactions and endocrine functions of these cells were discussed. By combined formaldehyde and chloral vapour treatment the cells of the pars intermedia exhibited a strong yellow fluorescence suggesting the presence of a peptide or peptides with NH2-terminal tryptophan. Masked metachromasia after acid hydrolysis was probably due to these peptides. Only a weak or no alpha-glycerophosphate dehydrogenase and nonspecific esterase activity was observed in the cells of pars intermedia compared to the cells of pars distalis suggesting low production rate of hormone synthesis. Specific and non-specific cholinesterases were demonstrated light and electron microscopically constantly in the cells bordering the lobules. These cells probably represent a certain type of glial cells. In the other cells the enzymatic activities varied markedly in intensity and distribution showing different ultrastructural localizations. Thus cholinesterase activities in the cells of pars intermedia reflect possibly different functional stages of the cells in their hormone production, storage and secretion processes.
对大鼠中间部细胞进行了光镜和电镜组织化学反应研究。讨论了这些细胞酶反应与内分泌功能之间可能的相关性。通过甲醛和氯醛蒸汽联合处理，中间部细胞呈现出强烈的黄色荧光，表明存在一种或多种具有NH2末端色氨酸的肽。酸水解后隐蔽的异染性可能归因于这些肽。与远侧部细胞相比，中间部细胞仅观察到微弱或无α-甘油磷酸脱氢酶和非特异性酯酶活性，提示激素合成的生产率较低。在小叶边界的细胞中，光镜和电镜下均持续显示出特异性和非特异性胆碱酯酶。这些细胞可能代表某种类型的神经胶质细胞。在其他细胞中，酶活性在强度和分布上有明显差异，显示出不同的超微结构定位。因此，中间部细胞中的胆碱酯酶活性可能反映了细胞在激素产生、储存和分泌过程中不同的功能阶段。
