Håkanson R, Sundler F, Larsson L I, Ekman R, Sjöberg N O
J Histochem Cytochem. 1975 Jan;23(1):65-74. doi: 10.1177/23.1.163855.
Fluorescence microscopy has demonstrated formaldehyde-ozone-induced fluorescence in the pars intermedia cells (melanocyte-stimulating hormone cells) and in certain cells of the pars distalis of the mammalian pituitary. From histochemical and chemical evidence the fluorescence is believed to reflect the presence of peptides with NH2-terminal tryptophan. In the pars distalis of hamster, cat and pig pituitary, the cells that exhibit formaldehyde-ozone-induced fluorescence have now been identified as adrenocorticotrophic hormone (ACTH) cells by immunohistochemistry. Granules from pig pituitaries were purified by passage through a succession of Millipore filters followed by centrifugation on a continuous sucrose gradient. Two granular fractions were identified by electron microscopy and found to contain high concentrations of peptides with NH2-terminal tryptophan as well as high ACTH bioactivity. These fractions, when pelleted and analyzed histochemically, displayed formaldehyde-ozone-induced fluorescence and ACTH-like immunoreactivity.
荧光显微镜检查已证实，甲醛-臭氧可在哺乳动物垂体的中间部细胞（促黑素细胞）和远侧部的某些细胞中诱导产生荧光。根据组织化学和化学证据，这种荧光被认为反映了具有NH2-末端色氨酸的肽的存在。在仓鼠、猫和猪垂体的远侧部，通过免疫组织化学现已确定，表现出甲醛-臭氧诱导荧光的细胞为促肾上腺皮质激素（ACTH）细胞。猪垂体颗粒先通过一系列密理博滤器，然后在连续蔗糖梯度上进行离心，从而得到纯化。通过电子显微镜鉴定出两个颗粒级分，发现它们含有高浓度的具有NH2-末端色氨酸的肽以及高ACTH生物活性。这些级分在沉淀并进行组织化学分析时，显示出甲醛-臭氧诱导的荧光和ACTH样免疫反应性。
