模拟失重对大鼠比目鱼肌肌梭中钙结合蛋白D-28K免疫反应性的影响。

Effects of simulated weightlessness on Calbindin D-28K-immunoreactivity in rat soleus muscle spindle.

作者信息

Wu Su-di, Fan Xiao-li, Tang Bin

机构信息

Department of Physiology, Xi'an Jiaotong University.

出版信息

Space Med Med Eng (Beijing). 2002 Oct;15(5):313-6.

PMID:12489518

Abstract

Objective. To analyze the mechanism involved in muscle spindle deterioration during simulated weightlessness. Method. Using the immunoperoxidase reaction utilizing the ABC (avidin-biotin-complex) method, Calbindin D28K-like immunoreactivity (CaBP-LI) of intrafusal fibres in soleus muscle were detected in 7 d, 14 d tail-suspended rats and compared with control rats. Result. The extrafusal muscle fibres and nerve fibres did not exhibit immunoreactivity to CaBP. CaBP-LI was found in some of the intrafusal muscle fibres in all the muscle spindles. After 14 d suspension, the immunoreactivity to CaBP of the intrafusal fibres decreased markedly. Conclusion. Simulated weightlessness could induce changes in the immunoreactivity of rat soleus muscle spindle to CaBP, which may contribute to the deterioration of muscle spindle.

摘要

目的。分析模拟失重期间肌梭退化所涉及的机制。方法。采用抗生物素蛋白-生物素复合物（ABC）法的免疫过氧化物酶反应，检测7天、14天尾部悬吊大鼠比目鱼肌梭内肌纤维的钙结合蛋白D28K样免疫反应性（CaBP-LI），并与对照大鼠进行比较。结果。梭外肌纤维和神经纤维对CaBP无免疫反应性。在所有肌梭的一些梭内肌纤维中发现了CaBP-LI。悬吊14天后，梭内纤维对CaBP的免疫反应性明显降低。结论。模拟失重可诱导大鼠比目鱼肌梭对CaBP的免疫反应性发生变化，这可能导致肌梭退化。