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从健康犬和患有外耳炎的犬分离出的凝固酶阳性葡萄球菌的鉴定及药敏试验

Identification and antimicrobial susceptibility of coagulase positive staphylococci isolated from healthy dogs and dogs suffering from otitis externa.

作者信息

Tejedor Junco M T, Martín Barrasa J L

机构信息

Departamento de Microbiología, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Canary Islands, Spain.

出版信息

J Vet Med B Infect Dis Vet Public Health. 2002 Nov;49(9):419-23. doi: 10.1046/j.1439-0450.2002.00571.x.

DOI:10.1046/j.1439-0450.2002.00571.x
PMID:12489709
Abstract

A total of 67 strains of coagulase positive staphylococci isolated from healthy dogs and dogs suffering from otitis externa were studied. Twenty-two isolates were from healthy dogs (five from hound dogs and 17 from companion dogs) and 45 from dogs suffering otitis externa (14 from hound dogs and 31 from companion dogs). Presumptive identification was attempted using the following tests: production of acetoin, anaerobic utilization of mannitol, acid production from mannitol, presence of beta-galactosidase, and growth on P agar supplemented with different concentrations of acriflavine. Susceptibility of staphylococci to 16 antibiotics was determined. Most effective antibiotics were imipenem, amoxycillin/clavulanic acid, ciprofloxacin, tobramycin, gentamicin and marbofloxacin. Penicillin, ampicillin and polymyxin B showed the lowest activity. There were no significant differences in antimicrobial susceptibility among isolates from healthy dogs and dogs suffering from otitis externa.

摘要

对从健康犬和患有外耳炎的犬分离出的67株凝固酶阳性葡萄球菌进行了研究。22株分离株来自健康犬(5株来自猎犬,17株来自宠物犬),45株来自患有外耳炎的犬(14株来自猎犬,31株来自宠物犬)。尝试使用以下试验进行初步鉴定:乙酰甲基甲醇的产生、甘露醇的厌氧利用、甘露醇产酸、β-半乳糖苷酶的存在以及在补充不同浓度吖啶黄的P琼脂上的生长情况。测定了葡萄球菌对16种抗生素的敏感性。最有效的抗生素是亚胺培南、阿莫西林/克拉维酸、环丙沙星、妥布霉素、庆大霉素和马波沙星。青霉素、氨苄青霉素和多粘菌素B活性最低。健康犬和患有外耳炎的犬的分离株之间在抗菌敏感性方面没有显著差异。

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