Zhang S C, Ghosh R, Jeske H
Department of Molecular Biology and Plant Virology, Institute of Biology, University of Stuttgart, Stuttgart, Germany.
Arch Virol. 2002 Dec;147(12):2349-63. doi: 10.1007/s00705-002-0880-9.
Abutilon mosaic geminivirus (AbMV) encodes two movement proteins, BV1 and BC1, which mediate the intra- and intercellular transport of viral DNA in plants cooperatively. It has been shown previously that singly expressed BC1, fused to green fluorescent protein (GFP), accumulates preferentially either at the cell periphery or around the nucleus in separate plant cells. To define the BC1 domains responsible for understanding the subcellular sorting, deletion mutants were fused to GFP and expressed transiently in epidermal cells of non-host (Allium cepa) as well as of host (Nicotiana benthamiana) plants with basically the same results in both species. BC1-mediated intracellular sorting was dependent on two protein domains, an "anchor domain" (amino acids 117 to 180) which is necessary and sufficient to fix GFP:BC1 at the cell periphery and the nuclear environment, and a "pilot domain" (amino acids 1 to 49) in the absence of which the fusion proteins were found at both sites in the same cell simultaneously.
苘麻花叶双生病毒（AbMV）编码两种运动蛋白，BV1和BC1，它们协同介导病毒DNA在植物细胞内和细胞间的运输。先前已经表明，单独表达的与绿色荧光蛋白（GFP）融合的BC1，在不同的植物细胞中优先积累在细胞周边或细胞核周围。为了确定负责亚细胞分选的BC1结构域，将缺失突变体与GFP融合，并在非寄主（洋葱）以及寄主（本氏烟草）植物的表皮细胞中瞬时表达，在这两个物种中得到的结果基本相同。BC1介导的细胞内分选依赖于两个蛋白质结构域，一个“锚定结构域”（氨基酸117至180），它对于将GFP:BC1固定在细胞周边和核环境中是必要且充分的，以及一个“引导结构域”（氨基酸1至49），在没有该结构域的情况下，融合蛋白会同时出现在同一细胞的两个位置。
