Le Bricon Thierry, Bengoufa Djaouida, Benlakehal Mourad, Bousquet Bernard, Erlich Danielle
Laboratoire de Biochimie A, Hôpital St-Louis AP-HP, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
Clin Biochem. 2002 Oct;35(7):565-7. doi: 10.1016/s0009-9120(02)00386-7.
Evaluate the Freelite free light chain immunoassay for urine analysis in myeloma. Urine specimens from 20 patients were analyzed by Freelite (The Binding Site) and SDS-agarose gel electrophoresis (Hydragel protéinurie, Sebia). Using the kappa/lambda ratio, Freelite was more sensitive than electrophoresis to detect free light chains, but concentration was overestimated in 75% of cases. Despite high sensitivity and full automation, Freelite inaccurately measures monoclonal free light chains in urine.
评估Freelite游离轻链免疫分析在骨髓瘤尿液分析中的应用。采用Freelite（The Binding Site公司）和SDS-琼脂糖凝胶电泳（Hydragel蛋白电泳，Sebia公司）对20例患者的尿液标本进行分析。利用κ/λ比值，Freelite在检测游离轻链方面比电泳更敏感，但在75%的病例中浓度被高估。尽管具有高灵敏度和全自动性，Freelite对尿液中单克隆游离轻链的测量仍不准确。
