Suppr超能文献

大鼠睾丸生精过程中促凋亡β-半乳糖苷结合凝集素半乳糖凝集素-1的调控表达及超微结构定位

Regulated expression and ultrastructural localization of galectin-1, a proapoptotic beta-galactoside-binding lectin, during spermatogenesis in rat testis.

作者信息

Dettin Luis, Rubinstein Natalia, Aoki Agustín, Rabinovich Gabriel A, Maldonado Cristina A

机构信息

Center of Electron Microscopy, School of Medical Sciences, National University of Cordoba, C.P. 5000, Córdoba, Argentina.

出版信息

Biol Reprod. 2003 Jan;68(1):51-9. doi: 10.1095/biolreprod.102.006361.

Abstract

Galectin-1, a highly conserved beta-galactoside-binding protein, induces apoptosis of activated T cells and suppresses the development of autoimmunity and chronic inflammation. To gain insight regarding the potential role of galectin-1 as a novel mechanism of immune privilege, we investigated expression and ultrastructural localization of galectin-1 in rat testis. Galectin-1 expression was assessed by Western blot analysis and immunocytochemical localization in testes obtained from rats aged from 9 to 60 days. Expression of this carbohydrate-binding protein was developmentally regulated, and its immunolabeling exhibited a stage-specific pattern throughout the spermatogenic process. Immunogold staining using the anti-galectin-1 antibody revealed the typical Sertoli cell profile in the seminiferous epithelium, mainly at stages X-II. During spermiation (stages VI-VIII), a strong labeling was observed at the luminal pole of seminiferous epithelium, localized on apical stalks of Sertoli cells, on heads of mature spermatids, and on bodies of residual cytoplasm. Moreover, spermatozoa released into the lumen showed a strong immunostaining. Following spermiation (stage VIII), galectin-1 expression was restored at the basal portion of Sertoli cells and progressively spread out through the whole cells as differentiation of germinal cells proceeded. Immunoelectron microscopy confirmed distribution of galectin-1 in nuclei and cytoplasmic projections of Sertoli cells and on heads and tails of late spermatids and residual bodies. Surface localization of galectin-1 was evidenced in spermatozoa from caput epididymis. Thus, the regulated expression of galectin-1 during the spermatogenic cycle suggests a novel role for this immunosuppressive lectin in reproductive biology.

摘要

半乳糖凝集素-1是一种高度保守的β-半乳糖苷结合蛋白,可诱导活化T细胞凋亡,并抑制自身免疫和慢性炎症的发展。为深入了解半乳糖凝集素-1作为免疫豁免新机制的潜在作用,我们研究了其在大鼠睾丸中的表达及超微结构定位。通过蛋白质免疫印迹分析和免疫细胞化学定位,对9至60日龄大鼠睾丸中的半乳糖凝集素-1表达进行了评估。这种碳水化合物结合蛋白的表达受发育调控,其免疫标记在整个生精过程中呈现出阶段特异性模式。使用抗半乳糖凝集素-1抗体进行免疫金染色,在生精上皮中显示出典型的支持细胞形态,主要在X-II期。在精子释放期(VI-VIII期),在生精上皮的管腔极观察到强烈的标记,定位于支持细胞的顶端柄、成熟精子细胞的头部以及残留细胞质体上。此外,释放到管腔中的精子显示出强烈的免疫染色。精子释放后(VIII期),随着生殖细胞的分化,半乳糖凝集素-1在支持细胞的基部恢复表达,并逐渐扩散至整个细胞。免疫电子显微镜证实半乳糖凝集素-1分布于支持细胞的细胞核和细胞质突起以及晚期精子细胞的头部和尾部及残留体上。在附睾头部的精子中证实了半乳糖凝集素-1的表面定位。因此,生精周期中半乳糖凝集素-1的调控表达表明这种免疫抑制凝集素在生殖生物学中具有新作用。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验