Reece Julie S, Miller Michael J, Arnold Mark A, Waterhouse Cris, Delaplaine Ted, Cohn Laura, Cannon Tom
Department of Chemistry, 230 IATL, The University of Iowa, Iowa City, IA 52242, USA.
Appl Biochem Biotechnol. 2003 Jan;104(1):1-11. doi: 10.1385/abab:104:1:1.
A compact, flow-through oxygen sensor device based on luminescence quenching was used to monitor dissolved oxygen levels during mammalian cell growth on the STS-93 mission of the Columbia space shuttle. Excitation of an oxygen-sensitive ruthenium complex was provided by a radioluminescent light source (0.9 mm in diameter, 2.5 mm long), and the intensity of the resulting luminescence was measured by a simple photodiode detector. The use of radioluminescence for the excitation light source is a unique approach that provides many features important for long-term and remote monitoring applications. For the spaceflight experiment, human lung fibroblast cells (WI-38) were grown in hollow-fiber bioreactors. Oxygen concentration was measured in the flow path both before and after the bioreactor cartridge in order to gain information about the metabolism of the cells. The sensor was found to be nonperturbing to cell growth and withstood the challenging physical conditions of shuttle launch and landing while maintaining a stable calibration function. In addition, the sensor provided physically meaningful oxygen predictions.
