Gao Frank G, Fay James M, Mathew Grace, Jeevarajan Antony S, Anderson Melody M
Wyle Life Sciences, 1290 Hercules Drive, Suite 120, Mail Stop BT-37, Houston, Texas 77058, USA.
J Biomed Opt. 2005 Sep-Oct;10(5):054005. doi: 10.1117/1.2062427.
There is a need to monitor the concentration of dissolved oxygen (DO) present in the culture medium for NASA's space cell biology experiments, as well as in earth-based cell cultures. Continuous measurement of DO concentration in the cell culture medium in perfused bioreactors requires that the oxygen sensor provide adequate sensitivity and low toxicity to the cells, as well as maintain calibration over several weeks. Although there are a number of sensors for dissolved oxygen on the market and under development elsewhere, very few meet these stringent conditions. An in-house optical oxygen sensor (HOXY) based on dynamic fluorescent quenching of Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) chloride and a pulsed blue LED light source was developed in our laboratory to address these requirements. The sensing element consisted of the fluorescent dye embedded in a silicone matrix and coated onto a glass capillary. Photobleaching was minimized by a pulsed LED light source. The total noise in the sensor output is 2% and the sensor dynamic range is 0 to 200 mm Hg. The resolution of the sensor is 0.1 mm Hg at 50 mm Hg, and 0.25 mm Hg at 130 mm Hg, while the accuracy is 5%. The LED-based oxygen sensor exhibited stable performance and low drift, making it compatible for space-flight bioreactor systems.
对于美国国家航空航天局（NASA）的太空细胞生物学实验以及地面细胞培养而言，有必要监测培养基中溶解氧（DO）的浓度。在灌注生物反应器中持续测量细胞培养基中的溶解氧浓度，要求氧气传感器对细胞具有足够的灵敏度和低毒性，并且能在数周内保持校准。尽管市场上有许多用于溶解氧的传感器，其他地方也在研发，但很少有能满足这些严格条件的。我们实验室开发了一种基于氯化三（4,7 - 二苯基 - 1,10 - 菲咯啉）钌（II）的动态荧光猝灭和脉冲蓝色LED光源的内部光学氧气传感器（HOXY），以满足这些要求。传感元件由嵌入硅树脂基质并涂覆在玻璃毛细管上的荧光染料组成。通过脉冲LED光源将光漂白降至最低。传感器输出的总噪声为2%，传感器动态范围为0至200毫米汞柱。该传感器在50毫米汞柱时的分辨率为0.1毫米汞柱，在130毫米汞柱时为0.25毫米汞柱，而精度为5%。基于LED的氧气传感器表现出稳定的性能和低漂移，使其适用于太空飞行生物反应器系统。
