霍乱毒素可诱导树突状细胞从上皮下圆顶区迁移至派尔集合淋巴结的T细胞区和B细胞区。
Cholera toxin induces migration of dendritic cells from the subepithelial dome region to T- and B-cell areas of Peyer's patches.
作者信息
Shreedhar Vijay K, Kelsall Brian L, Neutra Marian R
机构信息
GI Cell Biology Laboratory, Children's Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
Infect Immun. 2003 Jan;71(1):504-9. doi: 10.1128/IAI.71.1.504-509.2003.
Abstract
Intestinal M cells deliver macromolecules, particles, and pathogens into the subepithelial dome (SED) region of Peyer's patch mucosa, an area rich in dendritic cells (DCs). We tested whether uptake of the mucosal adjuvant cholera toxin (CT) or live Salmonella bacteria can induce DC migration within Peyer's patches. Virus-sized, fluorescent polystyrene microparticles were efficiently transported by M cells and ingested by CD11c(+), CD11b(-), and CD8a(-) DCs in the SED region. DCs loaded with microparticles remained in the SED for up to 14 days. CT (but not the CT B subunit) and live attenuated Salmonella enterica serovar Typhimurium bacteria induced migration of the microparticle-loaded DCs from the SED region into underlying B-cell follicles and adjacent parafollicular T-cell zones. Our data provide the first demonstration that DCs move in response to enterotoxin adjuvants and live bacteria that enter the mucosa via M cells.
摘要
肠道M细胞将大分子、颗粒和病原体递送至派尔集合淋巴结黏膜的上皮下圆顶（SED）区域，该区域富含树突状细胞（DC）。我们测试了黏膜佐剂霍乱毒素（CT）或活的沙门氏菌的摄取是否能诱导DC在派尔集合淋巴结内迁移。病毒大小的荧光聚苯乙烯微粒被M细胞有效转运，并被SED区域中的CD11c(+)、CD11b(-)和CD8a(-) DC摄取。负载微粒的DC在SED中停留长达14天。CT（而非CT B亚基）和减毒活的鼠伤寒沙门氏菌可诱导负载微粒的DC从SED区域迁移至下方的B细胞滤泡和相邻的滤泡旁T细胞区。我们的数据首次证明DC会响应肠毒素佐剂和通过M细胞进入黏膜的活细菌而移动。
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