Wang Yi Biao, Ogawa Yutaka, Doi Hideaki, Kusumoto Kenji, Jin Tie Nan, Ikehara Susumu
Department of Plastic and Reconstructive Surgery, Kansai Medical University, Moriguchi City, Osaka, Japan.
Plast Reconstr Surg. 2003 Jan;111(1):291-7; discussion 298-9. doi: 10.1097/01.PRS.0000035532.07303.9E.
The induction of donor-specific tolerance to skin allografts was investigated in rabbits using bone marrow transplantation techniques reported to be effective in mice. Various routes of bone marrow transplantation (i.e., intravenous, portal venous, or intraosseous) were also examined. In regimen A, the animals were treated with portal venous injection of bone marrow cells from the donor on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. In regimen B, the animals were treated with portal venous and intraosseous injections of donor bone marrow cells on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. In regimen C, the animals were given intraosseous injection of donor bone marrow cells on day 0 and intravenous injection of bone marrow cells from the same donor on posttransplant day 5. It was found that regimens B and C were more effective than regimen A in prolonging allograft survival. The results demonstrate that induction of allograft tolerance can be achieved by bone marrow transplantation in a rabbit model. This protocol deserves further study in other large animal models.
利用据报道在小鼠中有效的骨髓移植技术，在兔中研究了对皮肤同种异体移植物诱导供体特异性耐受的情况。还检查了各种骨髓移植途径（即静脉内、门静脉或骨内）。在方案A中，在第0天给动物门静脉注射来自供体的骨髓细胞，并在移植后第5天静脉注射来自同一供体的骨髓细胞。在方案B中，在第0天给动物门静脉和骨内注射供体骨髓细胞，并在移植后第5天静脉注射来自同一供体的骨髓细胞。在方案C中，在第0天给动物骨内注射供体骨髓细胞，并在移植后第5天静脉注射来自同一供体的骨髓细胞。发现方案B和C在延长同种异体移植物存活方面比方案A更有效。结果表明，在兔模型中通过骨髓移植可以实现同种异体移植物耐受的诱导。该方案值得在其他大型动物模型中进一步研究。
