Wang Yi Biao, Matusima Takasi, Kusumoto Kenji, Ogawa Yutaka
Department of Plastic and Reconstructive Surgery, kansai Medical University, Osaka, Japan.
Scand J Plast Reconstr Surg Hand Surg. 2005;39(2):73-6. doi: 10.1080/02844310410004955.
Previous studies in our laboratory have shown that giving bone marrow cells through the portal vein or intraosseous route is likely to be beneficial to tolerance of induction of allografts in rabbits. Using this model, we tested whether a less severe regimen for conditioning of the host can prevent rejection of allografts. Rabbits were given a single intraosseous injection of donor bone marrow cells and total body irradiation (7 Gy) and transplantation of skin and ear allografts. Mean skin allograft survival for this treatment was 88 days, which was similar to the results of our earlier study. A donor ear was accepted for more than a year with no signs of rejection. These results suggest that a single intraosseous injection of donor bone marrow cells is sufficient for induction of donor-specific tolerance in rabbits and that immunosuppressive agents may not be needed.
我们实验室之前的研究表明，通过门静脉或骨内途径给予骨髓细胞可能有利于诱导兔同种异体移植的耐受性。利用这个模型，我们测试了一种对宿主进行预处理的不太严格的方案是否可以预防同种异体移植的排斥反应。给兔子单次骨内注射供体骨髓细胞、全身照射（7 Gy）并移植皮肤和耳部同种异体移植物。这种治疗的皮肤同种异体移植物平均存活时间为88天，这与我们早期研究的结果相似。一个供体耳朵被接受了一年多，没有排斥迹象。这些结果表明，单次骨内注射供体骨髓细胞足以诱导兔产生供体特异性耐受性，可能不需要免疫抑制剂。
