Kirby Brian J, Shepodd Timothy J, Hasselbrink Ernest F
Microfluidics Department, Sandia National Laboratories, P.O. Box 969, MS 9951, Livermore, CA, USA.
J Chromatogr A. 2002 Dec 6;979(1-2):147-54. doi: 10.1016/s0021-9673(02)01453-x.
We present a microchip-based, voltage-addressable on/off valve architecture that is fundamentally consistent with the pressures and solvents employed for high-pressure liquid chromatography. Laser photopatterning of polymer monoliths inside glass microchannels is used to fabricate mobile fluid control elements, which are opened and closed by electrokinetic pressures. The glass substrates and crosslinked polymer monoliths operate in water-acetonitrile mixtures and have been shown to hold off pressures as high as 350 bar (5000 p.s.i.). Open/closed flow ratios of 10(4) to 10(6) have been demonstrated over the pressure range 1.5-70 bar (20-1000 p.s.i.), and the pressure-leak relationship shows the potential for valving control of flow through packed or monolithic chromatography columns. We expect that this valve platform will enable multiplexing of multiple chromatographic separations on single microchips.
我们展示了一种基于微芯片的、电压可寻址的开/关阀架构，该架构与用于高压液相色谱的压力和溶剂基本一致。利用玻璃微通道内聚合物整体的激光光刻图案来制造流动流体控制元件，这些元件通过电动压力打开和关闭。玻璃基板和交联聚合物整体可在水 - 乙腈混合物中运行，并且已证明能够承受高达350巴（5000磅力/平方英寸）的压力。在1.5 - 70巴（20 - 1000磅力/平方英寸）的压力范围内，已证明开/关流量比为10⁴至10⁶，并且压力 - 泄漏关系显示了对通过填充或整体式色谱柱的流动进行阀控的潜力。我们预计这种阀平台将能够在单个微芯片上实现多个色谱分离的多路复用。
