Guiochon Georges
Department of Chemistry, University of Tennessee, Knoxville, TN 37996-1600, USA.
J Chromatogr A. 2007 Oct 19;1168(1-2):101-68; discussion 100. doi: 10.1016/j.chroma.2007.05.090. Epub 2007 Jun 3.
Monolithic media have been used for various niche applications in gas or liquid chromatography for a long time. Only recently did they acquire a major importance in high-performance column liquid chromatography (HPLC). The advent of monolithic silica standard- and narrow-bore columns and of several families of polymer-based monolithic columns has considerably changed the HPLC field, particularly in the area of narrow-bore columns. The origin of the concept, the differences between their characteristics and those of traditional packed columns, their advantages and drawbacks, the methods of preparation of monoliths of different forms, and the current status of the field are reviewed. The actual and potential performance of monolithic columns are compared with those of packed columns. Monolithic columns have considerable advantages, which makes them most useful in many applications of liquid chromatography. They are extremely permeable and offer a high efficiency that decreases slowly with increasing flow velocity.
整体式介质长期以来一直用于气相或液相色谱的各种特定应用中。直到最近，它们才在高效柱液相色谱（HPLC）中变得至关重要。整体式硅胶标准内径和窄内径柱以及几类聚合物基整体柱的出现，极大地改变了HPLC领域，尤其是在窄内径柱领域。本文综述了整体柱概念的起源、其特性与传统填充柱特性的差异、优缺点、不同形式整体柱的制备方法以及该领域的现状。将整体柱的实际和潜在性能与填充柱进行了比较。整体柱具有相当大的优势，这使其在液相色谱的许多应用中非常有用。它们具有极高的渗透率，并且提供高效率，该效率随流速增加而缓慢降低。
