Chen Kuo-Hsing, Lin Ching-Erh, Liao Wei-Ssu, Lin Wann-Yin, Hsiao Yu-You
Department of Chemistry, National Taiwan University, P.O. Box 23-34, 1 Roosevelt Road Section 4, Taipei 10674, Taiwan.
J Chromatogr A. 2002 Dec 6;979(1-2):399-408. doi: 10.1016/s0021-9673(02)01501-7.
The influences of buffer pH and the concentration of beta-cyclodextrins (beta-CDs) on the separation and migration behavior of 13 structurally related phenothiazines in CD-modified capillary zone electrophoresis (CD-CZE) using a phosphate background electrolyte at low pH were investigated. We focused on the separation of these phenothiazines, including the enantiomers of chiral analytes, with the use of beta-CD and hydroxypropyl-beta-CD (HP-beta-CD) as electrolyte modifiers or chiral selectors at concentrations less than 8 mM. The results indicate that the interactions of phenothiazines with beta-CDs are very strong and that effective separations of 13 analytes can be achieved with addition of 0.3 mM beta-CD or 0.5 mM HP-beta-CD in a phosphate buffer at pH 3.0. Binding constants of phenothiazines to beta-CDs were evaluated for a better understanding of the interactions of phenothiazines with beta-CDs.
研究了缓冲液pH值和β-环糊精（β-CD）浓度对13种结构相关吩噻嗪在低pH值磷酸盐背景电解质的环糊精修饰毛细管区带电泳（CD-CZE）中的分离和迁移行为的影响。我们重点研究了这些吩噻嗪的分离，包括手性分析物的对映体，使用β-CD和羟丙基-β-环糊精（HP-β-CD）作为电解质改性剂或手性选择剂，浓度低于8 mM。结果表明，吩噻嗪与β-环糊精的相互作用非常强，在pH 3.0的磷酸盐缓冲液中添加0.3 mMβ-CD或0.5 mM HP-β-CD可以实现13种分析物的有效分离。评估了吩噻嗪与β-环糊精的结合常数，以更好地理解吩噻嗪与β-环糊精的相互作用。
