Ahmed Fayyaz, Wyckoff Jeffrey, Lin Elaine Y, Wang Weigang, Wang Yarong, Hennighausen Lothar, Miyazaki Jun-ichi, Jones Joan, Pollard Jeffrey W, Condeelis John S, Segall Jeffrey E
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Cancer Res. 2002 Dec 15;62(24):7166-9.
To examine the behavior of tumor cells in tumors developing directly from mammary tissue in transgenic models, we have evaluated transgenic mice expressing green fluorescent protein (GFP). Using the mouse mammary virus promoter (MMTV) to directly drive expression of GFP, we find low levels of fluorescence in the mammary and salivary glands of transgenic animals. Using MMTV-Cre or WAP-Cre in combination with the Cre-activatable CAG-CAT-EGFP construct, we find stronger expression of GFP that is still tissue specific. These animals provide a range of expression of GFP that is suitable for analysis of transgenic mammary tumors and metastases in vivo at the single cell level of resolution.
为了研究转基因模型中直接源自乳腺组织的肿瘤内肿瘤细胞的行为，我们评估了表达绿色荧光蛋白（GFP）的转基因小鼠。利用小鼠乳腺病毒启动子（MMTV）直接驱动GFP的表达，我们在转基因动物的乳腺和唾液腺中发现了低水平的荧光。将MMTV-Cre或WAP-Cre与可被Cre激活的CAG-CAT-EGFP构建体联合使用，我们发现GFP有更强的表达，且仍具有组织特异性。这些动物提供了一系列GFP表达情况，适用于在单细胞分辨率水平对转基因乳腺肿瘤及转移灶进行体内分析。
