Kadota Koji, Nishimura Shin-Ichiro, Bono Hidemasa, Nakamura Shugo, Hayashizaki Yoshihide, Okazaki Yasushi, Takahashi Katsutoshi
Computational Biology Research Center, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064 Japan.
Physiol Genomics. 2003 Feb 6;12(3):251-9. doi: 10.1152/physiolgenomics.00153.2002.
We applied a method based on Akaike's information criterion (AIC) to detect genes whose expression profile is considerably different in some tissue(s) than in others. Such observations are detected as outliers, and the method we used was originally developed to detect outliers. The main advantage of the method is that objective decisions are possible because the procedure is independent of a significance level. We applied the method to 48 expression ratios corresponding to various tissues in each of 14,610 clones obtained from the RIKEN Expression Array Database (READ; http://read.gsc.riken.go.jp). As a result, for several tissues (e.g., muscle, heart, and tongue tissues that contain similar cell types) we objectively obtained specific clones without any "thresholding." Our study demonstrates the feasibility of the method for detecting tissue-specific gene expression patterns.
