Luo Hailing, Kimura Koji, Aoki Mari, Hirako Makoto
Department of Animal Production, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Vet Med Sci. 2002 Nov;64(11):967-71. doi: 10.1292/jvms.64.967.
Three experiments were conducted to determine the effect of Vascular Endothelial Growth Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF(165) was employed at 5 ng/ml in modified synthetic oviduct fluid. In Exp. 1, bovine cumulus oocyte complexes were matured with or without VEGF for 22 hr, inseminated without VEGF for 6 hr, then cultured with or without VEGF for 42 hr. The cleavage rate and the development rate to 4- to 8-cell were higher (P<0.05) in groups with VEGF during in vitro maturation (IVM, 71.4% and 59.6%), in vitro culture (IVC, 70.3% and 62.3%), and both IVM and IVC (75.9% and 67.8%) than in the group cultured thoroughly without VEGF (49.9% and 38.4%, respectively). In Exp. 2, 4- to 8-cell embryos produced in vitro without VEGF were removed from cumulus cells at 48 hr post-insemination (Pi) and cultured with or without VEGF for 144 hr. The development rates to blastocyst at 96 hr (D6), 120 hr (D7) and 144 hr (D8) were similar (P>0.05) in both groups. In Exp. 3, cumulus cells were removed from presumptive embryos produced by IVM and IVF without VEGF at 10 hr Pi. Denuded embryos were cultured with or without VEGF for 38 hr or 182 hr. The cleavage rate and the development rates to 4- to 8-cell at 48 hr Pi and to blastocyst on D6, D7 and D8 were similar (P>0.05) in all groups. These results suggest that VEGF has a beneficial effect on the initial development of bovine embryo through surrounding cumulus cells.
进行了三项实验以确定血管内皮生长因子（VEGF）对体外培养的牛胚胎发育的影响。在改良的合成输卵管液中使用5 ng/ml的人重组VEGF(165)。在实验1中，牛卵丘卵母细胞复合体在有或无VEGF的情况下成熟22小时，在无VEGF的情况下受精6小时，然后在有或无VEGF的情况下培养42小时。在体外成熟（IVM，分别为71.4%和59.6%）、体外培养（IVC，分别为70.3%和62.3%）以及IVM和IVC（分别为75.9%和67.8%）过程中添加VEGF的组，其卵裂率和发育至4-8细胞的比率高于完全不添加VEGF培养的组（分别为49.9%和38.4%）（P<0.05）。在实验2中，在受精后48小时（Pi）将体外无VEGF条件下产生的4-8细胞胚胎从卵丘细胞中去除，并在有或无VEGF的情况下培养144小时。两组在96小时（D6）、120小时（D7）和144小时（D8）发育至囊胚的比率相似（P>0.05）。在实验3中，在Pi后10小时将体外成熟和体外受精无VEGF条件下产生的推测胚胎的卵丘细胞去除。去除卵丘的胚胎在有或无VEGF的情况下培养38小时或182小时。所有组在Pi后48小时的卵裂率以及发育至4-8细胞的比率，和在D6、D7和D8发育至囊胚的比率相似（P>0.05）。这些结果表明，VEGF通过包围卵丘细胞对牛胚胎的早期发育具有有益作用。
