Amini Mohammad Sobhan, Naderi Mohammad Mehdi, Shirazi Abolfazl, Aminafshar Mehdi, Boroujeni Sara Borjian, Pournourali Mostafa, Malekpour Ali
Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Avicenna J Med Biotechnol. 2022 Oct-Dec;14(4):287-293.
The aim of this study was to determine whether the addition of bioactive materials derived from Menstrual Blood Stem Cells (MenSCs) to the oocyte maturation medium may improve the quality of bovine embryos .
MenSCs were collected from 6 healthy women (between 26 and 36 years old) and after 3 days of culture, their bioactive materials were frozen. The bovine Cumulus-Oocyte-Complexes (COCs) were aspirated from ovarian slaughterhouse and the oocytes with more than three layers of cumulus cells were cultured in media supplemented with (treatment) and without (control) 10% MenSCs' bioactive materials. After IVM/IVF, the presumptive zygotes were cultured for 8 days.
The blastocyst rate on day 8 in treatment group was higher than control (40.2±1.9 . 23±4.2.3, p=0.001). The ratio of Trophectoderm (TE) and Inner Cell Mass (ICM) (ICM/TE) cells was also greater in treatment group compared to control (30.3±2 . 14.9±1; p=0.001). The re-expansion of vitrified blastocysts, 24 hours after warming, in treatment group was higher than control (93.3±2.5 . 66.2±8.8; p=0.01). The expression of some genes related to pluripotency and implantation (, , and ) were increased in treatment group compared to control (p<0/05).
In conclusion, the addition of MenSCs' bioactive materials during maturation of bovine oocytes could improve the quantity and quality of bovine IVP embryos. Also, the expression of some genes associated with pluripotency and implantation in the blastocyst would be increased.
本研究的目的是确定向卵母细胞成熟培养基中添加源自月经血干细胞（MenSCs）的生物活性物质是否可以提高牛胚胎的质量。
从6名健康女性（年龄在26至36岁之间）收集MenSCs，培养3天后，将其生物活性物质冷冻保存。从屠宰场采集牛的卵丘-卵母细胞复合体（COCs），将具有三层以上卵丘细胞的卵母细胞在添加（处理组）和不添加（对照组）10% MenSCs生物活性物质的培养基中培养。体外成熟/体外受精后，将假定的受精卵培养8天。
处理组第8天的囊胚率高于对照组（40.2±1.9对23±4.23，p=0.001）。与对照组相比，处理组的滋养外胚层（TE）和内细胞团（ICM）细胞比例（ICM/TE）也更高（30.3±2对14.9±1；p=0.001）。解冻后24小时，处理组玻璃化囊胚的再扩张率高于对照组（93.3±2.5对66.2±8.8；p=0.01）。与对照组相比，处理组中一些与多能性和着床相关的基因（、和）的表达增加（p<0.05）。
总之，在牛卵母细胞成熟过程中添加MenSCs的生物活性物质可以提高牛体外生产胚胎的数量和质量。此外，囊胚中一些与多能性和着床相关的基因表达也会增加。
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