Suzuki Motofumi, Nakase Takashi
Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), Wako 351-0198, Japan.
J Gen Appl Microbiol. 1999 Oct;45(5):239-246. doi: 10.2323/jgam.45.239.
To clarify phylogenetic relationships among species of the anamorphic ascomycetous genus Candida with ubiquinone Q-8, we determined complete sequences of 18S ribosomal RNA genes (18S rDNAs) from the type strains of 20 species of the genus Candida and 7 of the teleomorphic ascomycetous genera Pichia and Citeromyces, which have Q-8 as the major ubiquinone. Q-8-forming Candida species were divided into six clusters and were phylogenetically distant from a group of Candida species that included the type species of the genus. One Q-8-forming species from each of the genera Pichia, Citeromyces, or Clavispora was included in five of six clusters. Cluster 1 comprised C. ishiwadae, C. ernobii, C. karawaiewii, C. anatomiae, C. populi, and Pichia holstii. Cluster 2 comprised C. globosa and its teleomorph, Citeromyces matritensis. Cluster 3 comprised C. molischiana and Pichia capsulata. Cluster 4 comprised C. silvanorum, C. sequanensis, C. fennica, C. entomophila, C. homilentoma, C. rhagii, C. gotoi, and Pichia burtonii. Cluster 5 comprised C. fructus, C. musae, and C. lusitaniae (anamorph of Clavispora lusitaniae). Cluster 6 comprised C. stellata, C. lactiscondensi, C. galacta, and C. incommunis and was a heterogeneous group with large interspecific divergence. Pichia pastoris was quite divergent and phylogenetically distant from other Pichia species examined. Pichia methanolica and its synonym, P. cellobiosa, which have both Q-7 and Q-8 as major ubiquinones, were closely associated with Q-7-forming Williopsis salicorniae. Based on this comparative analysis of 18S rDNA sequences, it is evident that Q-8 Candida species and Q-8 Pichia species are polyphyletic.
为了阐明产泛醌Q-8的不完全型子囊菌念珠菌属各物种间的系统发育关系，我们测定了念珠菌属20个物种以及产孢型子囊菌毕赤酵母属和西特拉酵母属7个物种模式菌株的18S核糖体RNA基因（18S rDNA）的完整序列，这些物种均以Q-8作为主要泛醌。产Q-8的念珠菌物种被分为六个簇，并且在系统发育上与包括该属模式种在内的一组念珠菌物种相距甚远。毕赤酵母属、西特拉酵母属或克勒克酵母属中的一个产Q-8物种被纳入六个簇中的五个。簇1包括石和田念珠菌、厄氏念珠菌、卡拉威念珠菌、解剖学念珠菌、杨树念珠菌和霍氏毕赤酵母。簇2包括球形念珠菌及其有性型马特西特拉酵母。簇3包括莫利什念珠菌和荚膜毕赤酵母。簇4包括林地念珠菌、塞纳河念珠菌、芬兰念珠菌、嗜虫念珠菌、霍米伦托马念珠菌、拉吉念珠菌、戈托伊念珠菌和伯顿毕赤酵母。簇5包括果实念珠菌、香蕉念珠菌和葡萄牙念珠菌（葡糖假丝酵母的不完全型）。簇6包括星状念珠菌、乳酸凝聚念珠菌、半乳糖念珠菌和不常见念珠菌，是一个种间差异较大的异质类群。巴斯德毕赤酵母差异很大，在系统发育上与其他所检测的毕赤酵母物种相距甚远。甲醇毕赤酵母及其同义词纤维二糖毕赤酵母，同时以Q-7和Q-8作为主要泛醌，与产Q-7的盐沼威克酵母密切相关。基于对18S rDNA序列的这种比较分析，很明显产Q-8的念珠菌物种和产Q-8的毕赤酵母物种是多系的。
