Rapid microsatellite analysis using discontinuous polyacrylamide gel electrophoresis.
作者信息
White E, Sahota R, Edes S
机构信息
Pacific Forestry Centre, Canadian Forest Service, 506 West Burnside Road, Victoria, BC V8Z 1M5, Canada.
出版信息
Genome. 2002 Dec;45(6):1107-9. doi: 10.1139/g02-084.
DOI:10.1139/g02-084
PMID:12502255
Abstract
A method for screening large numbers of samples for microsatellites using discontinuous, non-denaturing polyacrylamide gels and rapid fluorescent gel staining is described. Disc electrophoresis on slab gels provides high-resolution of PCR products. It is useful for collecting population data once microsatellite loci have been characterized.
摘要
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