LIN-39/Hox触发细胞分裂并抑制EFF-1/融合素依赖性外阴细胞融合。
LIN-39/Hox triggers cell division and represses EFF-1/fusogen-dependent vulval cell fusion.
作者信息
Shemer Gidi, Podbilewicz Benjamin
机构信息
Department of Biology, Technion-Israel Institute of Technology, Haifa, 32000, Israel.
出版信息
Genes Dev. 2002 Dec 15;16(24):3136-41. doi: 10.1101/gad.251202.
Abstract
General mechanisms by which Hox genes establish cell fates are known. However, a few Hox effectors mediating cell behaviors have been identified. Here we found the first effector of LIN-39/HoxD4/Dfd in Caenorhabditis elegans. In specific vulval precursor cells (VPCs), LIN-39 represses early and late expression of EFF-1, a membrane protein essential for cell fusion. Repression of eff-1 is also achieved by the activity of CEH-20/Exd/Pbx, a known cofactor of Hox proteins. Unfused VPCs in lin-39(-);eff-1(-) double mutants fail to divide but migrate, executing vulval fates. Thus, lin-39 is essential for inhibition of EFF-1-dependent cell fusion and stimulation of cell proliferation during vulva formation. Supplemental material is available at http://www.genesdev.org.
摘要
Hox基因建立细胞命运的一般机制已为人所知。然而，介导细胞行为的少数Hox效应因子已被鉴定出来。在这里，我们在秀丽隐杆线虫中发现了LIN-39/HoxD4/Dfd的首个效应因子。在特定的外阴前体细胞（VPCs）中，LIN-39抑制EFF-1的早期和晚期表达，EFF-1是一种对细胞融合至关重要的膜蛋白。CEH-20/Exd/Pbx（一种已知的Hox蛋白辅因子）的活性也能实现对eff-1的抑制。lin-39(-);eff-1(-)双突变体中未融合的VPCs无法分裂但能迁移，执行外阴命运。因此，lin-39对于抑制外阴形成过程中EFF-1依赖的细胞融合和刺激细胞增殖至关重要。补充材料可在http://www.genesdev.org获取。
相似文献
1
LIN-39/Hox triggers cell division and represses EFF-1/fusogen-dependent vulval cell fusion.LIN-39/Hox触发细胞分裂并抑制EFF-1/融合素依赖性外阴细胞融合。
Genes Dev. 2002 Dec 15;16(24):3136-41. doi: 10.1101/gad.251202.
2
Cell fates and fusion in the C. elegans vulval primordium are regulated by the EGL-18 and ELT-6 GATA factors -- apparent direct targets of the LIN-39 Hox protein.秀丽隐杆线虫外阴原基中的细胞命运和融合由EGL-18和ELT-6 GATA因子调控，它们显然是LIN-39 Hox蛋白的直接靶标。
Development. 2002 Nov;129(22):5171-80. doi: 10.1242/dev.129.22.5171.
3
Transcriptional control of Notch signaling by a HOX and a PBX/EXD protein during vulval development in C. elegans.秀丽隐杆线虫外阴发育过程中HOX蛋白和PBX/EXD蛋白对Notch信号通路的转录调控
Dev Biol. 2007 Feb 15;302(2):661-9. doi: 10.1016/j.ydbio.2006.09.049. Epub 2006 Oct 4.
4
The C. elegans hox gene lin-39 controls cell cycle progression during vulval development.秀丽隐杆线虫的同源基因lin-39在阴门发育过程中控制细胞周期进程。
Dev Biol. 2016 Oct 1;418(1):124-134. doi: 10.1016/j.ydbio.2016.07.018. Epub 2016 Jul 27.
5
Competence and commitment of Caenorhabditis elegans vulval precursor cells.秀丽隐杆线虫外阴前体细胞的能力与承诺。
Dev Biol. 1999 Aug 1;212(1):12-24. doi: 10.1006/dbio.1999.9357.
6
Transcriptional upregulation of the C. elegans Hox gene lin-39 during vulval cell fate specification.秀丽隐杆线虫Hox基因lin-39在阴门细胞命运特化过程中的转录上调。
Mech Dev. 2006 Feb;123(2):135-50. doi: 10.1016/j.mod.2005.11.003. Epub 2006 Jan 18.
7
Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6.多种转录因子直接调控秀丽隐杆线虫胚胎和幼虫腹侧皮下细胞中的Hox基因lin-39表达，包括皮下命运调控因子LIN-26和ELT-6。
BMC Dev Biol. 2014 May 13;14:17. doi: 10.1186/1471-213X-14-17.
8
Caenorhabditis elegans HOM-C genes regulate the response of vulval precursor cells to inductive signal.秀丽隐杆线虫同源异型基因（HOM-C）调控外阴前体细胞对诱导信号的反应。
Dev Biol. 1997 Feb 1;182(1):150-61. doi: 10.1006/dbio.1996.8471.
9
Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction.在秀丽隐杆线虫外阴诱导过程中，Wnt信号的激活绕过了对RTK/Ras信号的需求。
Genes Dev. 2002 May 15;16(10):1281-90. doi: 10.1101/gad.981602.
10
The Pristionchus HOX gene Ppa-lin-39 inhibits programmed cell death to specify the vulva equivalence group and is not required during vulval induction.小杆线虫HOX基因Ppa-lin-39抑制程序性细胞死亡以确定外阴等价组，并且在外阴诱导过程中不是必需的。
Development. 1998 Oct;125(19):3865-73. doi: 10.1242/dev.125.19.3865.
引用本文的文献
1
Prolonging somatic cell proliferation through constitutive hox gene expression in C. elegans.通过在秀丽隐杆线虫中组成性表达 hox 基因来延长体细胞增殖。
Nat Commun. 2023 Oct 27;14(1):6850. doi: 10.1038/s41467-023-42644-1.
2
Mechanisms of lineage specification in Caenorhabditis elegans.线虫中谱系特化的机制。
Genetics. 2023 Dec 6;225(4). doi: 10.1093/genetics/iyad174.
3
Identifying the Caenorhabditis elegans vulval transcriptome.鉴定秀丽隐杆线虫的生殖转录组。
G3 (Bethesda). 2022 May 30;12(6). doi: 10.1093/g3journal/jkac091.
4
The anterior Hox gene ceh-13 and elt-1/GATA activate the posterior Hox genes nob-1 and php-3 to specify posterior lineages in the C. elegans embryo.线虫胚胎中，前 Hox 基因 ceh-13 和 elt-1/GATA 激活后 Hox 基因 nob-1 和 php-3 以特化胚胎的后段谱系。
PLoS Genet. 2022 May 2;18(5):e1010187. doi: 10.1371/journal.pgen.1010187. eCollection 2022 May.
5
hsp-90 and unc-45 depletion induce characteristic transcriptional signatures in coexpression cliques of C. elegans.热休克蛋白 90 和 UNC-45 耗竭诱导秀丽隐杆线虫共表达聚类中特征性的转录特征。
Sci Rep. 2021 Jun 18;11(1):12852. doi: 10.1038/s41598-021-91690-6.
6
Variability in β-catenin pulse dynamics in a stochastic cell fate decision in C. elegans.线虫中β-连环蛋白在随机细胞命运决定中的脉冲动力学变化。
Dev Biol. 2020 May 15;461(2):110-123. doi: 10.1016/j.ydbio.2020.02.004. Epub 2020 Feb 4.
7
How cells fuse.细胞如何融合。
J Cell Biol. 2019 May 6;218(5):1436-1451. doi: 10.1083/jcb.201901017. Epub 2019 Apr 1.
8
Auto-fusion and the shaping of neurons and tubes.神经元和神经管的自动融合与塑形。
Semin Cell Dev Biol. 2016 Dec;60:136-145. doi: 10.1016/j.semcdb.2016.07.018. Epub 2016 Jul 18.
9
RAB-5- and DYNAMIN-1-Mediated Endocytosis of EFF-1 Fusogen Controls Cell-Cell Fusion.RAB-5和动力蛋白-1介导的EFF-1融合素内吞作用控制细胞间融合。
Cell Rep. 2016 Feb 16;14(6):1517-1527. doi: 10.1016/j.celrep.2016.01.027. Epub 2016 Feb 4.
10
A model of the regulatory network involved in the control of the cell cycle and cell differentiation in the Caenorhabditis elegans vulva.一种参与秀丽隐杆线虫外阴细胞周期控制和细胞分化的调控网络模型。
BMC Bioinformatics. 2015 Mar 13;16:81. doi: 10.1186/s12859-015-0498-z.
本文引用的文献
1
Control of vulval competence and centering in the nematode Oscheius sp. 1 CEW1.线虫Oscheius sp. 1 CEW1中阴门感受态的调控与定位
Genetics. 2003 Jan;163(1):133-46. doi: 10.1093/genetics/163.1.133.
2
Cell fusion: an EFFicient sculptor.细胞融合：一位高效的塑造者。
Curr Biol. 2002 Jul 9;12(13):R467-9. doi: 10.1016/s0960-9822(02)00948-x.
3
The zinc finger protein REF-2 functions with the Hox genes to inhibit cell fusion in the ventral epidermis of C. elegans.锌指蛋白REF-2与Hox基因共同作用，抑制秀丽隐杆线虫腹侧表皮中的细胞融合。
Development. 2002 Jul;129(14):3335-48. doi: 10.1242/dev.129.14.3335.
4
Activation of Wnt signaling bypasses the requirement for RTK/Ras signaling during C. elegans vulval induction.在秀丽隐杆线虫外阴诱导过程中，Wnt信号的激活绕过了对RTK/Ras信号的需求。
Genes Dev. 2002 May 15;16(10):1281-90. doi: 10.1101/gad.981602.
5
Changing potency by spontaneous fusion.通过自发融合改变效力。
Nature. 2002 Apr 4;416(6880):545-8. doi: 10.1038/nature729. Epub 2002 Mar 13.
6
The type I membrane protein EFF-1 is essential for developmental cell fusion.I型膜蛋白EFF-1对发育过程中的细胞融合至关重要。
Dev Cell. 2002 Mar;2(3):355-62. doi: 10.1016/s1534-5807(02)00129-6.
7
Cooperative regulation of AJM-1 controls junctional integrity in Caenorhabditis elegans epithelia.AJM-1的协同调节控制秀丽隐杆线虫上皮细胞的连接完整性。
Nat Cell Biol. 2001 Nov;3(11):983-91. doi: 10.1038/ncb1101-983.
8
Functional comparison of the nematode Hox gene lin-39 in C. elegans and P. pacificus reveals evolutionary conservation of protein function despite divergence of primary sequences.秀丽隐杆线虫和太平洋杆线虫中线虫Hox基因lin-39的功能比较揭示了尽管一级序列存在差异，但蛋白质功能具有进化保守性。
Genes Dev. 2001 Aug 15;15(16):2161-72. doi: 10.1101/gad.200601.
9
REF-1, a protein with two bHLH domains, alters the pattern of cell fusion in C. elegans by regulating Hox protein activity.REF-1是一种具有两个bHLH结构域的蛋白质，它通过调节Hox蛋白的活性来改变秀丽隐杆线虫中的细胞融合模式。
Development. 2001 May;128(10):1793-804. doi: 10.1242/dev.128.10.1793.
10
Overlapping roles of two Hox genes and the exd ortholog ceh-20 in diversification of the C. elegans postembryonic mesoderm.两个Hox基因和同源基因ceh-20在秀丽隐杆线虫胚胎后中胚层多样化中的重叠作用。
Development. 2000 Dec;127(23):5179-90. doi: 10.1242/dev.127.23.5179.
Zotero 插件