Molenkamp Richard, Kooi Engbert A, Lucassen Marjoleine A, Greve Sophie, Thijssen Joyphi C P, Spaan Willy J M, Bredenbeek Peter J
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, The Netherlands.
J Virol. 2003 Jan;77(2):1644-8. doi: 10.1128/jvi.77.2.1644-1648.2003.
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
构建了嵌合黄热病毒(YF)RNA,其中YF结构基因被丙型肝炎病毒(HCV)结构基因取代,或被YF与HCV结构基因之间的融合基因取代。有趣的是,RNA复制需要位于3'端的保守序列1与位于YF衣壳序列5'端的RNA序列之间的核苷酸互补性。(嵌合)HCV结构蛋白能有效表达和加工,并形成天然的E1/E2异二聚体。然而,未获得产生HCV样颗粒的迹象。