丙型肝炎病毒样颗粒的形态发生
Hepatitis C virus-like particle morphogenesis.
作者信息
Blanchard Emmanuelle, Brand Denys, Trassard Sylvie, Goudeau Alain, Roingeard Philippe
机构信息
Laboratoire de Virologie, E3M-EA3250, IFR 82, Faculté de Médecine et Centre Hospitalier Universitaire, Tours, France.
出版信息
J Virol. 2002 Apr;76(8):4073-9. doi: 10.1128/jvi.76.8.4073-4079.2002.
Abstract
Although much is known about the hepatitis C virus (HCV) genome, first cloned in 1989, little is known about HCV structure and assembly due to the lack of an efficient in vitro culture system for HCV. Using a recombinant Semliki forest virus replicon expressing genes encoding HCV structural proteins, we observed for the first time the assembly of these proteins into HCV-like particles in mammalian cells. This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions.
摘要
尽管人们对1989年首次克隆的丙型肝炎病毒（HCV）基因组了解很多，但由于缺乏用于HCV的高效体外培养系统，对HCV的结构和组装知之甚少。利用表达编码HCV结构蛋白基因的重组Semliki森林病毒复制子，我们首次观察到这些蛋白在哺乳动物细胞中组装成HCV样颗粒。该系统为研究病毒形态发生和病毒-宿主细胞相互作用开辟了新的可能性。
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