氢氧化铝佐剂制备过程中的热处理对老化过程中蛋白质吸附能力的影响。

Effect of thermal treatment during the preparation of aluminum hydroxide adjuvant on the protein adsorption capacity during aging.

作者信息

Dandashli Euphemie A, Zhao Qinjian, Yitta Silaja, Morefield Garry L, White Joe L, Hem Stanley L

机构信息

Department of Industrial and Physical Pharmacy, School of Pharmacy, Purdue University, West Lafayette, IN, USA.

出版信息

Pharm Dev Technol. 2002 Nov;7(4):401-6. doi: 10.1081/pdt-120015042.

DOI:10.1081/pdt-120015042

PMID:12503522

Abstract

Six aluminum hydroxide adjuvants, poorly crystalline aluminum oxyhydroxide (AlOOH) were prepared using different thermal treatments of amorphous aluminum hydroxide (Al(OH)3) in an effort to increase the protein adsorption capacity. All of the adjuvants initially exhibited a higher protein adsorption capacity. However, the protein adsorption capacity decreased during aging at room temperature. X-ray and differential centrifugal sedimentation analysis revealed that complete dehydration of amorphous aluminum hydroxide to aluminum oxyhydroxide is required to produce a stable adjuvant. Any residual amorphous aluminum hydroxide will spontaneously transform to crystalline aluminum hydroxide during aging at room temperature. Since crystalline aluminum hydroxide has a small surface area, the protein adsorption capacity of adjuvants containing amorphous aluminum hydroxide decreased by 30-40% when stored for 6 months at room temperature.

摘要

制备了六种氢氧化铝佐剂，即通过对无定形氢氧化铝（Al(OH)₃）进行不同的热处理得到的 poorly crystalline aluminum oxyhydroxide（AlOOH），以提高蛋白质吸附能力。所有佐剂最初都表现出较高的蛋白质吸附能力。然而，在室温老化过程中蛋白质吸附能力下降。X射线和差示离心沉降分析表明，无定形氢氧化铝完全脱水形成氢氧化氧铝才能产生稳定的佐剂。任何残留的无定形氢氧化铝在室温老化过程中都会自发转化为结晶氢氧化铝。由于结晶氢氧化铝的表面积较小，含有无定形氢氧化铝的佐剂在室温下储存6个月后，其蛋白质吸附能力下降了30 - 40%。