鸡基因组中组织特异性组蛋白H5基因的重复频率。

Reiteration frequency of the gene for tissue-specific histone H5 in the chicken genome.

作者信息

Scott A C, Wells J R

出版信息

Nature. 1976 Feb 26;259(5545):635-8. doi: 10.1038/259635a0.

Abstract

Chicken erythroid cells contain a tissue specific histone known as H5 in addition to the five major histone species found in other organisms. The mRNA coding for this histone has been isolated by indirect immunoprecipitation from immature, non-dividing reticulocytes in which this is the only histone synthesised. The mRNA has been modified by the enzymatic addition of a 3' polyadenylic acid tract, and transcribed into complementary DNA (cDNA) using the RNA-dependent DNA-polymerase from avian myeloblastosis virus. Studies on the hybridisation of this cDNA indicate that the gene coding for the H5 histone is reiterated 10 times in the chicken genome.

摘要

鸡的红细胞除了含有其他生物体中发现的五种主要组蛋白外，还含有一种被称为H5的组织特异性组蛋白。编码这种组蛋白的mRNA已通过间接免疫沉淀从未成熟的、不分裂的网织红细胞中分离出来，在这些细胞中这是唯一合成的组蛋白。该mRNA已通过酶促添加3'聚腺苷酸尾进行了修饰，并使用禽成髓细胞瘤病毒的RNA依赖性DNA聚合酶转录成互补DNA（cDNA）。对该cDNA杂交的研究表明，编码H5组蛋白的基因在鸡基因组中重复了10次。