Ruiz-Carrillo A, Affolter M, Renaud J
J Mol Biol. 1983 Nov 15;170(4):843-59. doi: 10.1016/s0022-2836(83)80191-0.
The organization of the genes coding for histones in the chicken has been examined, with special reference to that coding for the tissue-specific, developmentally regulated histone H5. Two recombinant phages containing sequences complementary to cloned H5 cDNA have been isolated from a genomic chicken library. The clones have been characterized by heteroduplex formation, restriction nuclease analysis, hybridization to cloned homologous histone gene probes, and DNA sequencing. Hybridization to genomic DNA has shown that there is only one copy of the H5 gene per haploid genome, whereas there are six to 11 copies of the genes for the other histones. Examination of 29 X 10(3) base-pairs of DNA sequences flanking the H5 gene has revealed the absence of any other histone genes which, although not tandemly reiterated, for the most part appear to reside in loosely organized clusters. The complete DNA sequence of the H5 gene and flanking regions, as well as the mapping of the 5'-end of its messenger RNA by primer extension with AMV reverse transcriptase, has shown that the gene has no introns and little homology to other histone genes, including those for H1.
已对鸡中编码组蛋白的基因组织进行了研究，特别参考了编码组织特异性、发育调控的组蛋白H5的基因。从鸡基因组文库中分离出了两个与克隆的H5 cDNA互补序列的重组噬菌体。这些克隆已通过异源双链体形成、限制性核酸酶分析、与克隆的同源组蛋白基因探针杂交以及DNA测序进行了表征。与基因组DNA杂交表明，单倍体基因组中每个H5基因只有一个拷贝，而其他组蛋白基因有6到11个拷贝。对H5基因两侧29×10³个碱基对的DNA序列进行检查发现，没有任何其他组蛋白基因，这些基因虽然不是串联重复的，但在很大程度上似乎位于松散组织的簇中。H5基因及其侧翼区域的完整DNA序列，以及通过AMV逆转录酶进行引物延伸对其信使RNA 5′端的定位，表明该基因没有内含子，与其他组蛋白基因几乎没有同源性，包括H1的基因。
