Zhang Ping, Quinton Lee J, Bagby Gregory J, Summer Warren R, Nelson Steve
Department of Medicine, Section of Pulmonary/Critical Care Medicine, Louisiana State University Health Sciences Center, New Orleans 70112, USA.
J Infect Dis. 2003 Jan 1;187(1):62-9. doi: 10.1086/346027. Epub 2002 Dec 13.
CXC chemokines are major chemoattractants for pulmonary polymorphonuclear leukocyte (PMNL) recruitment. To study the effects of interferon (IFN)-gamma on the pulmonary chemokine response to lipopolysaccharide (LPS) challenge, rats were treated with intratracheal IFN-gamma (1x10(5) U/rat) 24 h before an intratracheal LPS (100 microg/rat) challenge. Intratracheal LPS caused significant increases in both cytokine-induced neutrophil chemoattractant (CINC) and macrophage inflammatory protein-2 in bronchoalveolar lavage (BAL) fluid and pulmonary PMNL recruitment. IFN-gamma enhanced these responses. IFN-gamma also increased LPS-induced tumor necrosis factor (TNF)-alpha in BAL fluid. LPS-induced TNF-alpha and CINC mRNA expression in alveolar macrophages was increased by IFN-gamma. CD11b/c and CD18 expression on circulating PMNLs was not affected by IFN-gamma, nor was the chemotaxis of these cells. IFN-gamma increases the pulmonary CXC chemokine response, which may serve as one mechanism underlying enhanced PMNL delivery into the lung.
CXC趋化因子是肺部多形核白细胞（PMNL）募集的主要趋化因子。为研究干扰素（IFN）-γ对肺部趋化因子对脂多糖（LPS）刺激反应的影响，在气管内给予LPS（100μg/只大鼠）刺激前24小时，给大鼠气管内注射IFN-γ（1×10⁵U/只大鼠）。气管内给予LPS导致支气管肺泡灌洗（BAL）液中细胞因子诱导的中性粒细胞趋化因子（CINC）和巨噬细胞炎性蛋白-2以及肺部PMNL募集显著增加。IFN-γ增强了这些反应。IFN-γ还增加了BAL液中LPS诱导的肿瘤坏死因子（TNF）-α。IFN-γ增加了肺泡巨噬细胞中LPS诱导的TNF-α和CINC mRNA表达。循环PMNL上的CD11b/c和CD18表达不受IFN-γ影响，这些细胞的趋化性也不受影响。IFN-γ增加肺部CXC趋化因子反应，这可能是增强PMNL向肺内输送的一种潜在机制。
