胃蛋白酶原C基因多态性与胃癌遗传易感性之间的关联
Association between pepsinogen C gene polymorphism and genetic predisposition to gastric cancer.
作者信息
Liu Hui-Jie, Guo Xiao-Lin, Dong Ming, Wang Lan, Yuan Yuan
机构信息
Cancer Institute, First Affiliated Hospital, China Medical University, Shenyang 110001, Liaoning Province, China.
出版信息
World J Gastroenterol. 2003 Jan;9(1):50-3. doi: 10.3748/wjg.v9.i1.50.
AIM
To identify a molecular marker for gastric cancer, and to investigate the relationship between the polymorphism of pepsinogen C (PGC) gene and the genetic predisposition to gastric cancer.
METHODS
A total of 289 cases were involved in this study. 115 cases came from Shenyang area, a low risk area of gastric cancer, including 42 unrelated controls and 73 patients with gastric cancer. 174 cases came from Zhuanghe area, a high-risk area of gastric cancer, including 113 unrelated controls, and 61 cases from gastric cancer kindred families. The polymorphism of PGC gene was detected by polymerase chain reaction (PCR) and the relation between the genetic polymorphism of PGC and gastric cancer was examined.
RESULTS
Four alleles, 31 0bp (allele 1), 400 bp (allele 2), 450 bp (allele 3), and 480 bp (allele 4) were detected by PCR. The frequency of allele 1 was higher in patients with gastric cancer than that in controls. Genotypes containing homogenous allele 1 were significantly more frequent in patients with gastric cancer than that in controls (0.33, 0.14, chi(2)=3.86, P<0.05). There was no significant difference between the control group of Zhuanghe and the group of gastric cancer kindred. But the frequency of allele 1 was higher in control group of Zhuanghe area than that in control group of Shenyang area and genotypes containing homogenous allele 1 were significantly more frequent in the control group of Zhuanghe area than those in control group of Shenyang area (0.33, 0.14, chi(2)=4.32, P<0.05). In the group of gastric cancer kindred the frequency of allele 1 was significantly higher than that in control group of Shenyang area (0.5164, 0.3571, chi(2)=4.47, P<0.05). Genotypes containing homogenous allele 1 were significantly more frequent in the group of gastric cancer kindred than those in control group of Shenyang area (0.36, 0.14, chi(2)=4.91, P<0.05).
CONCLUSION
These results suggest that there is some relation between pepsinogen C gene polymorphism and gastric cancer, and the person with homogenous allele 1 predisposes to gastric cancer than those with other genotypes. Pepsinogen C gene polymorphism may be used as a genetic marker for a genetic predisposition to gastric cancer. The distribution of pepsinogen C gene polymorphism in Zhuanghe, a high-risk area of gastric cancer, is different from that in Shenyang, a low risk area of gastric cancer.
目的
鉴定一种胃癌分子标志物，研究胃蛋白酶原C（PGC）基因多态性与胃癌遗传易感性之间的关系。
方法
本研究共纳入289例。115例来自胃癌低发区沈阳地区，包括42例无关对照和73例胃癌患者。174例来自胃癌高发区庄河地区，包括113例无关对照和61例胃癌家族成员。采用聚合酶链反应（PCR）检测PGC基因多态性，并分析PGC基因多态性与胃癌的关系。
结果
通过PCR检测到4个等位基因，分别为310bp（等位基因1）、400bp（等位基因2）、450bp（等位基因3）和480bp（等位基因4）。胃癌患者中，等位基因1的频率高于对照组。胃癌患者中含纯合等位基因1的基因型频率显著高于对照组（0.33，0.14，χ2 = 3.86，P < 0.05）。庄河对照组与胃癌家族组之间无显著差异。但庄河地区对照组中等位基因1的频率高于沈阳地区对照组，庄河地区对照组中含纯合等位基因1的基因型频率显著高于沈阳地区对照组（0.33，0.14，χ2 = 4.32，P < 0.05）。在胃癌家族组中，等位基因1的频率显著高于沈阳地区对照组（0.5164，0.3571，χ2 = 4.47，P < 0.05）。胃癌家族组中含纯合等位基因1的基因型频率显著高于沈阳地区对照组（0.36，0.14，χ2 = 4.91，P < 0.05）。
结论
这些结果提示胃蛋白酶原C基因多态性与胃癌之间存在一定关系，纯合等位基因1的个体比其他基因型个体更易患胃癌。胃蛋白酶原C基因多态性可作为胃癌遗传易感性的遗传标志物。胃癌高发区庄河地区胃蛋白酶原C基因多态性的分布与胃癌低发区沈阳地区不同。
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