Xu Qian, Liu Jing-wei, He Cai-yun, Sun Li-ping, Gong Yue-hua, Jing Jing-Jing, Xing Cheng-zhong, Yuan Yuan
Tumor Etiology and Screening Department of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China ; Key Laboratory of Cancer Etiology and Prevention, China Medical University, Liaoning Provincial Education Department, Shenyang, Liaoning, People's Republic of China.
PLoS One. 2014 Feb 25;9(2):e89203. doi: 10.1371/journal.pone.0089203. eCollection 2014.
The aim of this study was to investigate the interaction effects of pri-let-7a-1 rs10739971 with pepsinogen C (PGC) and excision repair cross complementing group 6 (ERCC6) gene polymorphisms and its association with the risks of gastric cancer and atrophic gastritis. We hoped to identify miRNA polymorphism or a combination of several polymorphisms that could serve as biomarkers for predicting the risk of gastric cancer and its precancerous diseases.
Sequenom MassARRAY platform method was used to detect polymorphisms of pri-let-7a-1 rs10739971 G → A, PGC rs4711690 C → G, PGC rs6458238 G → A, PGC rs9471643 G → C, and ERCC6 rs1917799 in 471 gastric cancer patients, 645 atrophic gastritis patients and 717 controls.
An interaction effect of pri-let-7a-1 rs10739971 polymorphism with ERCC6 rs1917799 polymorphism was observed for the risk of gastric cancer (P interaction = 0.026); and interaction effects of pri-let-7a-1 rs10739971 polymorphism with PGC rs6458238 polymorphism (P interaction = 0.012) and PGC rs9471643 polymorphism (P interaction = 0.039) were observed for the risk of atrophic gastritis.
The combination of pri-let-7a-1 rs10739971 polymorphism and ERCC6 and PGC polymorphisms could provide a greater prediction potential than a single polymorphism on its own. Large-scale studies and molecular mechanism research are needed to confirm our findings.
本研究旨在探讨前体微小RNA-let-7a-1 rs10739971与胃蛋白酶原C（PGC）及切除修复交叉互补基因6（ERCC6）基因多态性的相互作用及其与胃癌和萎缩性胃炎风险的关联。我们希望确定可作为预测胃癌及其癌前疾病风险生物标志物的微小RNA多态性或几种多态性的组合。
采用Sequenom MassARRAY平台方法检测471例胃癌患者、645例萎缩性胃炎患者和717例对照者的前体微小RNA-let-7a-1 rs10739971 G→A、PGC rs4711690 C→G、PGC rs6458238 G→A、PGC rs9471643 G→C以及ERCC6 rs1917799的多态性。
观察到前体微小RNA-let-7a-1 rs10739971多态性与ERCC6 rs1917799多态性对胃癌风险存在相互作用（交互作用P = 0.026）；观察到前体微小RNA-let-7a-1 rs10739971多态性与PGC rs6458238多态性（交互作用P = 0.012）以及PGC rs9471643多态性（交互作用P = 0.039）对萎缩性胃炎风险存在相互作用。
前体微小RNA-let-7a-1 rs10739971多态性与ERCC6和PGC多态性的组合比单一多态性具有更大预测潜力。需要大规模研究和分子机制研究来证实我们的发现。
