Rassulin Iu Iu, Fedorova N A, Rechinskiĭ V O, Parfanovich M I
Vopr Virusol. 1988 Jan-Feb;33(1):58-63.
The method allowing the detection of BLV proviral DNA in the peripheral blood leukocytes of cattle is reported. Cell DNA from leukocytes used without preliminary cultivation was dot-hydridized with 32P-labeled plasmid that included a fragment of BLV proviral DNA. In parallel, sera from the cattle under study were tested by immunodiffusion assay (ID). The results indicate that dot-hybridization assay is more sensitive as a diagnostic test than ID because it detects BLV infection in apparently normal cattle which was seronegative by ID.
报道了一种检测牛外周血白细胞中牛白血病病毒（BLV）前病毒DNA的方法。未经过初步培养的白细胞的细胞DNA与包含BLV前病毒DNA片段的32P标记质粒进行斑点杂交。同时，采用免疫扩散试验（ID）检测所研究牛的血清。结果表明，斑点杂交试验作为一种诊断测试比免疫扩散试验更敏感，因为它能检测出免疫扩散试验血清学阴性的看似正常的牛中的BLV感染。
