通过等电聚焦从纯化的大鼠胰腺细胞核中分离组蛋白组分。

Segregation of histone fractions from purified rat pancreas nuclei by isoelectric focusing.

作者信息

Kopelovich L, Wolfe G, Pfeffer L

出版信息

Proc Soc Exp Biol Med. 1976 Feb;151(2):400-6. doi: 10.3181/00379727-151-39220.

Abstract

The present report describes the purification of nuclei from rat pancreas and the use of isoelectric focusing for the segregation of histone fractions, a heterogeneous mixture with respect to charge. This procedure employs gels of 5% polyacrylamide as support matrices, in 6.25 M urea, and spans a pH range of 7.5-10.8. It was, therefore, found adequate for study of the microheterogeneity of histones. The banding pattern of histone fractions in pancreas development using electrofocusing is presented.

摘要

本报告描述了从大鼠胰腺中纯化细胞核以及使用等电聚焦法分离组蛋白组分的过程，组蛋白组分是一种电荷不均一的混合物。该方法采用5%聚丙烯酰胺凝胶作为支持基质，在6.25M尿素中，pH范围为7.5 - 10.8。因此，发现该方法适用于研究组蛋白的微观异质性。文中呈现了使用电聚焦法观察到的胰腺发育过程中组蛋白组分的条带模式。