Thomas-Hall Skye, Watson Kenneth, Scorzetti Gloria
Int J Syst Evol Microbiol. 2002 Nov;52(Pt 6):2303-2308. doi: 10.1099/00207713-52-6-2303.
A morphological and physiological characterization of yeast strains CBS 8908, CBS 8915, CBS 8920, CBS 8925(T) and CBS 8926, isolated from Antarctic soils, was performed. Phylogenetic analyses of the sequences of the D1/D2 regions and the adjacent internal transcribed spacer (ITS) regions of the large-subunit rDNA of these strains placed them into the Tremellales clade of the Hymenomycetes. The sequence data identified strains CBS 8908, CBS 8915 and CBS 8920 as belonging to the species Cryptococcus victoriae. Strains CBS 8925(T) and CBS 8926 were found to represent an unique clade within the Hymenomycetes, with Dioszegia crocea CBS 6714(T) being their closest phylogenetic relative. Fatty acid composition and proteome fingerprint data for these novel strains were also obtained. No sexual state was observed. A novel basidiomycetous species, Cryptococcus statzelliae, is proposed for strains CBS 8925(T) and CBS 8926.
对从南极土壤中分离出的酵母菌株CBS 8908、CBS 8915、CBS 8920、CBS 8925(T)和CBS 8926进行了形态学和生理学特征分析。对这些菌株大亚基核糖体DNA的D1/D2区域及相邻的内转录间隔区(ITS)区域序列进行系统发育分析,结果将它们归入担子菌纲的银耳目分支。序列数据确定菌株CBS 8908、CBS 8915和CBS 8920属于维多利亚隐球菌物种。发现菌株CBS 8925(T)和CBS 8926代表担子菌纲内一个独特的分支,其中克罗伊西亚地丝酵母CBS 6714(T)是它们亲缘关系最近的系统发育相关菌株。还获得了这些新菌株的脂肪酸组成和蛋白质组指纹数据。未观察到有性状态。为菌株CBS 8925(T)和CBS 8926提出了一个新的担子菌物种,即施塔策尔隐球菌。