Banerjee D, Mandal A, Mukherjee S
Department of Physical Chemistry, Indian Association for the Cultivation of Science, 2A & B Raja SC Mullick Road, Jadavpur, Calcutta 700032, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2003 Jan 1;59(1):103-9. doi: 10.1016/s1386-1425(02)00125-7.
Fluorescence quenching of some important aromatic bio-molecules (ABM) such as 3-aminophthalhydrazide (luminol), tryptophan (Try), phenylalanine and tyrosine (Tyr) by methyl glyoxal (MG) has been studied employing different spectroscopic techniques. The interaction of MG with ABM in the excited state has been analysed using Stern-Volmer (S-V) mechanism. In the case of MG-luminol system time correlated single photon counting (TCSPC) technique has also been applied to explain the S-V mechanism. The bimolecular rate constants obtained are found to be higher than the rate constant for diffusion controlled process. A plausible explanation of the quenching mechanism has been discussed on the basis of hydrogen bonding, charge transfer and energy transfer interaction between the colliding species.
利用不同的光谱技术研究了甲基乙二醛（MG）对一些重要的芳香族生物分子（ABM）如3-氨基邻苯二甲酰肼（鲁米诺）、色氨酸（Try）、苯丙氨酸和酪氨酸（Tyr）的荧光猝灭。采用斯特恩-沃尔默（S-V）机理分析了MG与ABM在激发态下的相互作用。在MG-鲁米诺体系中，还应用了时间相关单光子计数（TCSPC）技术来解释S-V机理。发现得到的双分子速率常数高于扩散控制过程的速率常数。基于碰撞物种之间的氢键、电荷转移和能量转移相互作用，对猝灭机理进行了合理的解释。
