Canuel Clelia, Badre Sophie, Groenzin Henning, Berheide Markus, Mullins Oliver C
Schlumberger-Doll Research, Ridgefield, Connecticut 06877, USA.
Appl Spectrosc. 2003 May;57(5):538-44. doi: 10.1366/000370203321666560.
The quenching of the fluorescence of five aromatic hydrocarbons by three halogenated organics and by molecular oxygen has been measured. Both fluorescence intensity and fluorescence lifetime measurements have been employed to validate results and interpretation; linear Stern-Volmer analyses are shown to apply throughout. The fluorescence quenching rate constant of molecular oxygen for the five aromatic hydrocarbons is essentially equivalent to the diffusion rate constant independent of the fluorophore excitation energy. The halogenated organic-fluorophore rate constants vary by a factor of 965 and are shown to correlate roughly with the energy difference between the quencher and fluorophore excited electronic states in accord with a standard model of quantum two-level mixing. The value of the coupling interaction energy is approximately 2500 cm(-1).
已测量了三种卤代有机物和分子氧对五种芳烃荧光的猝灭情况。同时采用荧光强度和荧光寿命测量来验证结果及解释；结果表明线性斯特恩-沃尔默分析在整个过程中均适用。分子氧对这五种芳烃的荧光猝灭速率常数基本上等同于扩散速率常数，且与荧光团激发能无关。卤代有机物与荧光团的速率常数相差965倍，并且根据量子双能级混合的标准模型，显示出与猝灭剂和荧光团激发电子态之间的能量差大致相关。耦合相互作用能的值约为2500 cm(-1)。
