[烧伤后早期肥厚性瘢痕相关细胞骨架基因表达的研究]
[A study of the expression of hypertrophic scar related cytoskeletal genes during early postburn stage].
作者信息
Ma Bing, Wu Jun, Yi Shaoxuan, Wang Zhenxiang, He Weifeng, Zhu Jin, Luo Gaoxing, Chen Xiwei
机构信息
Institute of Burn Research, Southwestern Hospital, The Third Military Medical University, Chongqing 400038, P.R. China.
出版信息
Zhonghua Shao Shang Za Zhi. 2002 Feb;18(1):29-31.
OBJECTIVE
To screen the hypertrophic scar related cytoskeletal genes during early postburn stage, so as to explore their roles in postburn scar contraction.
METHODS
cDNA microarray chips containing 4096 human cDNAs were employed to investigate the cytoskeletal gene expression of the scar samples from human postburn hypertrophic scar. Furthermore, the expression of one of the cytoskeletal genes in hypertrophic scar tissue was studied by in situ hybridization.
RESULTS
Thirteen up - regulated cytoskeletal genes in 3 early postburn hypertrophic scar samples were identified. Moreover, the cells expressing human tropomyosin TM30 mRNA, one of the up - regulated cytoskeletal genes, were found increased in the early postburn hypertrophic scar samples.
CONCLUSION
In this study up - regulated expression of many hypertrophic scar related cytoskeletal genes was found in the scar samples during early postburn stage, and they might be important factors leading to postburn hypertrophic scar formation and contraction.
目的
筛选烧伤后早期阶段与增生性瘢痕相关的细胞骨架基因，以探讨其在烧伤后瘢痕挛缩中的作用。
方法
采用包含4096个人类cDNA的cDNA微阵列芯片，研究人烧伤后增生性瘢痕瘢痕样本的细胞骨架基因表达。此外，通过原位杂交研究增生性瘢痕组织中一种细胞骨架基因的表达。
结果
在3个烧伤后早期增生性瘢痕样本中鉴定出13个上调的细胞骨架基因。此外，在烧伤后早期增生性瘢痕样本中发现，表达上调的细胞骨架基因之一人原肌球蛋白TM30 mRNA的细胞增多。
结论
本研究发现，在烧伤后早期阶段，瘢痕样本中许多与增生性瘢痕相关的细胞骨架基因表达上调，它们可能是导致烧伤后增生性瘢痕形成和挛缩的重要因素。
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