Kitade Tatsuya, Konda Hisashi, Takegami Shigehiko, Ishii Kumiyo, Ishikawa Chika, Kitamura Keisuke
Kyoto Pharmaceutical University, Kyoto, Japan.
Chem Pharm Bull (Tokyo). 2003 Jan;51(1):53-7. doi: 10.1248/cpb.51.53.
An easy and sensitive method for the quantitative determination of ofloxacin (OFLX), a new fluoroquinolone antimicrobial agent, in a pharmaceutical formulation, tablet, was developed by using solid-substrate room-temperature phosphorimetry (RTP) on a poly(vinyl alcohol) substrate. The method did not require a dry gas flush during the measurement of phosphorescence. The influence of different conditions such as solution pH and concentrations of heavy atoms, used as the enhancer, were studied. The phosphorescence intensity of OFLX was enhanced using NaOH and KI as enhancers. A linear relationship between concentration and RTP intensity for each standard solution was obtained in the concentration range of 4-18000 ng/ml, and the determination limit was 4 ng/ml. The proposed method was applied to a determination of OFLX in a commercial tablet, and the results were compared with those of fluorescence and UV methods. It was proven that OFLX in a commercial tablet can be accurately measured by this method with a very small amount of sample solution.
采用聚乙烯醇基质上的固体基质室温磷光法（RTP），开发了一种简便、灵敏的方法用于定量测定药物制剂片剂中的新型氟喹诺酮抗菌剂氧氟沙星（OFLX）。该方法在磷光测量过程中无需干燥气体冲洗。研究了溶液pH值和用作增强剂的重原子浓度等不同条件的影响。以NaOH和KI作为增强剂可增强OFLX的磷光强度。在4-18000 ng/ml的浓度范围内，各标准溶液的浓度与RTP强度之间呈线性关系，测定限为4 ng/ml。将该方法应用于市售片剂中OFLX的测定，并将结果与荧光法和紫外法的结果进行比较。结果表明，用极少量的样品溶液，该方法就能准确测定市售片剂中的OFLX。
