纸基质室温磷光法测定药物制剂中的硫鸟嘌呤

Determination of thioguanine in pharmaceutical preparations by paper substrate room temperature phosphorimetry.

作者信息

Chuan D, Wen Y, Shaomin S, Pin Y

机构信息

Institute of Molecular Science, Shanxi University, Taiyuan, China.

出版信息

Analyst. 2000 Jul;125(7):1327-30. doi: 10.1039/b001597k.

DOI:10.1039/b001597k

PMID:10984930

Abstract

A room temperature phosphorimetric (RTP) procedure was used for the determination of 6-thioguanine (6-TG). The method is based on paper substrate room temperature phosphorimetry (PS-RTP) using indium sulfate, In2(SO4)3 as a heavy atom perturber. Various factors affecting the room temperature phosphorescence of 6-TG are discussed. The linear dynamic range for 6-TG is from 3.3 to 334.3 ng per spot with a detection limit of 4.6 ng per spot and a relative standard deviation (RSD) of 2.38%. The recovery of standard 6-TG added to commercial tablets is in the range 96.39-98.44%. The method is simple, rapid and sensitive and can be applied to the analysis of commercial tablets without interference.

摘要

采用室温磷光法（RTP）测定6-硫鸟嘌呤（6-TG）。该方法基于以硫酸铟（In2(SO4)3）作为重原子微扰剂的纸基质室温磷光法（PS-RTP）。讨论了影响6-TG室温磷光的各种因素。6-TG的线性动态范围为每斑点3.3至334.3 ng，检测限为每斑点4.6 ng，相对标准偏差（RSD）为2.38%。添加到市售片剂中的标准6-TG的回收率在96.39 - 98.44%范围内。该方法简单、快速且灵敏，可用于市售片剂的分析且无干扰。