Stadler H, Mondon M, Ziegler C
University of Kaiserslautern, Department of Physics, Erwin Schroedinger Str. 56, 67663 Kaiserslautern, Germany.
Anal Bioanal Chem. 2003 Jan;375(1):53-61. doi: 10.1007/s00216-002-1664-5. Epub 2002 Nov 28.
Adsorption of the protein bovine serum albumin (BSA) on gold has been tested at various concentrations in aqueous solution by dynamic contact-angle analysis (DCA) and quartz-crystal microbalance (QCM) measurements. With the Wilhelmy plate technique advancing and receding contact angles and the corresponding hysteresis were measured and correlated with the hydrophilicity and the homogeneity of the surface. With electrical admittance measurements of a gold-coated piezoelectrical quartz crystal, layer mass and viscoelastic contributions to the resonator's frequency shift during adsorption could be separated. A correlation was found between the adsorbed mass and the homogeneity and hydrophilicity of the adsorbed film.
通过动态接触角分析（DCA）和石英晶体微天平（QCM）测量，在水溶液中不同浓度下测试了蛋白质牛血清白蛋白（BSA）在金上的吸附情况。采用Wilhelmy板技术测量了前进接触角和后退接触角以及相应的滞后现象，并将其与表面的亲水性和均匀性相关联。通过对镀金压电石英晶体的电导测量，可以分离出吸附过程中膜质量和粘弹性对谐振器频率偏移的贡献。发现吸附质量与吸附膜的均匀性和亲水性之间存在相关性。
